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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Retinoblastoma Gene Protein (pSer608) Antibody, clone 51B7    

Anti-Retinoblastoma Gene Protein (pSer608) Antibody, clone 51B7

  • WB - Anti-Retinoblastoma Gene Protein (pSer608) Antibody, clone 51B7  ABD11700
    NALM6 lysate probed with Mouse anti Retinoblastoma Gene Protein ABD11700
  • WB - Anti-Retinoblastoma Gene Protein (pSer608) Antibody, clone 51B7  ABD11700
    Western blot of recombinant RB. E. Human RB protein was purified from E. coli and either Mock phosphorylated or phosphorylated in vitro using recombinant Cyclin D1/ CDK4 complex (D1/K4) or Cyclin E/ CDK2 complex (E/K2). The blot was probed with ABD11700 recognizing Rb phosphorylated in residue S608 . The blot was stripped and subsequently counter-probed with pan Rb antiserum
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06400
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 51B7
Calculated MW 106159 Da
Additional Information
Other Species H,M,Eastern Newt (Notophthalmus viridescens)
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Synthetic peptide C-ADMYLSPLRSPK phosphorylated at S608, conugated to KLH.
Shelf Life 18 months from date of despatch.
Gene ID 5925
Other Names Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Target/Specificity Mouse anti-retinoblastoma gene protein (pSer608), clone 51B7 recognizes human and mouse Rb protein only when it has been phosphorylated at the S608 residue.Mouse anti-retinoblastoma gene protein (pSer608), clone 51B7 does not recognize non-phosphorylated Rb or Rb phosphorylated at other sites.Phosphorylation of the Rb protein at S608 is dependent upon CDK4 (Zarkowska and Mittnacht 1997).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Retinoblastoma Gene Protein (pSer608) Antibody, clone 51B7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Barrie, S. et al. (2003) High-throughput screening for the identification of small-molecule inhibitors of retinoblastoma protein phosphorylation in cells.
Anal. Biochem. 320:66-74. 2. Bettencourt-Dias, M. et al. (2003) Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes.
J Cell Sci. 116: 4001-9.

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Cat# ABD11700
(40 western blots)
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