|Application ||FC, E, ELISPOT|
|Calculated MW||20438 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Plasmid cDNA encoding bovine IL-10.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10|
|Target/Specificity||Mouse anti-Bovine Interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti-Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Interleukin-10 Antibody, clone CC318 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.Vet Immunol Immunopathol. 85 (3-4): 213-23. 2. Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models andmacrophages.Infect Immun. 78: 1383-9. 3. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.J Leukoc Biol. 83 (1): 48-55. 4. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells. Int Arch Allergy Immunol. 144: 325-37. 5. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70. 6. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.Funct Integr Genomics. 10: 21-38. 7. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.Clin Experiment Ophthalmol. 38: 405-13. 8. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19. 9. Bannerman, D.D. et al.(2004)Escherichia coliandStaphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72. 10. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.Vet Res. 41: 14. 11. den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.PLoSone 6: e18188 12. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.Vet Res. 44: 59. 13. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705. 14. Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.Clin Vaccine Immunol. 17: 1946-51. 15. McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.Immunology. 139: 227-44. 16. Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.PLoS One. 7: e30430. 17. Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease.Clin Vaccine Immunol. 18: 1467-76. 18. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48. 19. Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.PLoS One. 10 (5): e0124144.
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