|Application ||WB, IHC-P|
|Calculated MW||28999 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant human Granzyme A.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granzyme A, 220.127.116.11, CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF, GZMA, CTLA3, HFSP|
|Target/Specificity||Mouse anti-Human granzyme A antibody, clone GA6 recognizes Granzyme A, a ~60 kDa disulphide-linked homodimeric protein of two 262 amino acid chains, expressed in cytoplasmic granules of cytotoxic lymphocytes and NK cells.Granzyme A is involved in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice are indistinguishable from normal animals in their response to infection.Granzyme A has been proposed as a potential biomarker for patients with active tuberculosis with significantly lower levels present in the plasma of patients with the active form of the disease compared to patients with latent infection (Gugginoet al.2015)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Granzyme A Antibody, clone GA6 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Kummer, J. A. et al. (1993) Production and characterisation of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins. J. Immunol. Methods. 163: 77-83. 2. Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 12: 567-77. 3. Meade, J.L. et al. (2009) Proteolytic activation of the cytotoxic phenotype during human NK cell development.J Immunol. 183: 803-13. 4. Hochegger, K. et al. (2007) Expression of granzyme A in human polymorphonuclear neutrophils.Immunology. 121: 166-73. 5. Grodzovski, I. et al. (2011) IL-2-granzyme A chimeric protein overcomes multidrug resistance (MDR) through a caspase 3-independent apoptotic pathway.Int J Cancer. 128: 1966-80. 6. Suck, G. et al. (2005) KHYG-1, a model for the study of enhanced natural killer cell cytotoxicity.Exp Hematol. 33: 1160-71. 7. Vrazo, A.C. et al. (2015) Live cell evaluation of granzyme delivery and death receptor signaling in tumor cells targeted by human natural killer cells.Blood. 126 (8): e1-e10.
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