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Anti-Granzyme B Antibody, clone GB10 (Biotin)

Mouse Anti-Human Monoclonal Antibody

  • E - Anti-Granzyme B Antibody, clone GB10 (Biotin) ABD11706
    Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human plasma samples are diluted i:2 (green) and 1:4 (blue)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10144
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names GB10
Calculated MW 27716 Da
Additional Information
Other Species Chimpanzee,Pr,Rh
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Purified human Granzyme B.
Shelf Life 18 months from date of despatch.
Gene ID 3002
Other Names Granzyme B,, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB
Target/Specificity Mouse anti-Human granzyme B antibody, clone GB10 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Granzyme B Antibody, clone GB10 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Spaeny-Dekking, E. H. A. et al. (1998) Extracellular Granzymes A and B in humans: Detection of native species during CTL responses in vitroandin vivo.
J. Immunol. 160: 3610 - 3616.

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Cat# ABD11706
(40 western blots)
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