|Application ||IHC-F, IF, FC, E, IP|
|Calculated MW||27716 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Purified human Granzyme B.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granzyme B, 22.214.171.124, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB|
|Target/Specificity||Mouse anti-Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.Mouse anti-Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Granzyme B Antibody, clone GB11 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Spaeny-Dekking, E.H. et al. (1998) Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo.J Immunol. 160 (7): 3610-6. 2. Tschopp, C.M. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma. Blood.108:2290-9. 3. Wever, D. C. et al. (1998) The CD8+ Granzyme B+ T cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. Immunol. 93: 383 4. Hsi, B.L. & Yeh, C.J. (1986) Monoclonal antibodies to human amnion.J Reprod Immunol. 9 (1): 11-21. 5. Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 12: 567-77. 6. Davis, C.C. et al. (2010) Interleukin-7 permits Th1/Tc1 maturation and promotes ex vivo expansion of cord blood T cells: a critical step toward adoptive immunotherapy after cord blood transplantation.Cancer Res. 70: 5249-58. 7. Hallermalm, K. et al. (2008) Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes.J Immunol. 180: 3766-74. 8. Hufner, K. et al. (2009) Fewer latent herpes simplex virus type 1 and cytotoxic T cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve.J Virol. 83: 3696-703. 9. Kumar, D. et al. (2009) JNK MAPK pathway regulates constitutive transcription of CCL5 by human NK cells through SP1.J Immunol. 182: 1011-20. 10. Clayton, A. et al. (2008) Human tumor-derived exosomes down-modulate NKG2D expression.J Immunol. 180: 7249-58. 11. Schleypen, J.S. et al. (2006) Cytotoxic markers and frequency predict functional capacity of natural killer cells infiltrating renal cell carcinoma.Clin Cancer Res. 12: 718-25. 12. Hodge, S. et al. (2015) Increased CD8 T-cell granzyme B in COPD is suppressed by treatment with low-dose azithromycin.Respirology. 20 (1): 95-100.
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