|Application ||IHC-F, FC, IP|
|Calculated MW||28147 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Shelf Life||18 months from date of despatch.|
|Other Names||Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD58, CD58, LFA3|
|Target/Specificity||Mouse anti-Human CD58 antibody, clone MEM-63 recognizes human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Macgoba et al. 1989). CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD58 Antibody, clone MEM-63 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Makgoba, M.W. et al. (1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.Immunology Today 10: 417-422. 2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716. 3. Grundy, J.E. et al. (1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.Immunology. 78 (3): 413-20. 4. Megyola, C. et al. (2011) Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.Virol J. 8: 84.
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