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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD58 Antibody, clone MEM-63    

Anti-Human CD58 Antibody, clone MEM-63

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD58 Antibody, clone MEM-63  ABD11709
    Staining of human peripheral blood platelets with Mouse anti Human CD58:FITC
  • FC - Anti-Human CD58 Antibody, clone MEM-63  ABD11709
    Staining of human peripheral blood monocytes with Mouse anti Human CD58: Azide Free
  • FC - Anti-Human CD58 Antibody, clone MEM-63  ABD11709
    Published customer image:Mouse anti Human CD58 antibody, clone MEM-63 used for the evaluation of CD58 expression on B cells by flow cytometry.Image caption:CD23hiProliferating CD23hiCD58+ cells and non-proliferating CD23loCD58+ cells express EBV latency genes. A. EBV-exposed B cells were FACS-sorted on day 4 into regions R1 (CD23loCD58-), R2 (CD23loCD58+), and R3 (CD23hiCD58+). Sorting strategy and purity of each population are shown. Regions were drawn with spaces in between to prevent contamination between regions. B. The relative transcript levels of latency genes EBNA1, LMP1, and EBNA2 in each of the three sorted sub-populations were determined by real-time reverse transcription-PCR with gene-specific primers. RNA from already immortalized cells from the same subject was used as positive control while RNA from un-infected PBMC from the same subject was used as negative control. C. EBV-exposed B cells were harvested on day 3 followed by staining for CD23 , CD58 , and intracellular LMP1 and flow cytometry. Expression of LMP1 in cells gated on R1, R2, and R3 is shown. Percentages represent fractions of gated cells expressing LMP1 or detected by the corresponding isotype control antibody. D. Sorted CD27+ memory and CD27- naïve B cells as in the experiment shown in Fig. 5B were exposed to EBV, harvested on day 5, and examined for expression of intracellular IL6 and LMP1 . Numbers represent percentages of CD27+ memory or CD27- naïve B cells.From: Megyola C, Ye J, Bhaduri-McIntosh S.Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.Virol J. 2011 Feb 25;8:84.
  • FC - Anti-Human CD58 Antibody, clone MEM-63  ABD11709
    Staining of human peripheral blood granulocytes with Mouse anti Human CD58:RPE
  • FC - Anti-Human CD58 Antibody, clone MEM-63  ABD11709
    Published customer image:Mouse anti Human CD58 antibody, clone MEM-63 used for the evaluation of CD58 expression on B cells by flow cytometry.Image caption:CD23hiCD58+ cells do not proliferate in the absence of EBV-exposed non-proliferating sub-populations of cells. Three days after exposure of CD3-depleted B cells to EBV, CD23hiCD58+ cells, representing 0.5% of the culture (A) were FACS-sorted. Post-sort analysis of CD23hiCD58+ cells is shown in B. Sorted CD23hiCD58+ cells were mixed with un-infected autologous primary B cells (as feeder cells) and re-introduced into culture at 0.5% of the total culture. Mock-sorted cells were also re-introduced into culture as control. Cells were harvested four days later and stained for CD23 and CD58 . Un-infected cells (C), EBV-exposed cells (D), mock-sorted but EBV-exposed cells (E), and sorted-CD23hiCD58+ cells mixed with un-infected B cells (F) after a total of 7 days in culture are shown. Percentages represent fraction of CD23hiCD58+ cells out of total.From: Megyola C, Ye J, Bhaduri-McIntosh S.Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.Virol J. 2011 Feb 25;8:84.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC, IP
Primary Accession P19256
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-63
Calculated MW 28147 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Shelf Life 18 months from date of despatch.
Gene ID 965
Other Names Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD58, CD58, LFA3
Target/Specificity Mouse anti-Human CD58 antibody, clone MEM-63 recognizes human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Macgoba et al. 1989). CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD58 Antibody, clone MEM-63 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Makgoba, M.W. et al. (1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.
Immunology Today 10: 417-422. 2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716. 3. Grundy, J.E. et al. (1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.
Immunology. 78 (3): 413-20. 4. Megyola, C. et al. (2011) Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.
Virol J. 8: 84.

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