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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human CD326 Antibody, clone MOC-31    

Anti-Human CD326 Antibody, clone MOC-31

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Human CD326 Antibody, clone MOC-31  ABD11714
    Published customer image:Mouse anti Human CD326 antibody used for the detection of CD326 positive cells in epithlial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:EpEx immunohistochemical analysis in epithelial cancers control tissues. The negative control photomicrographs are shown. HNSCC (A), prostate cancer (B), colon cancer (C), breast cancer (D), ESCC (E) and normal esophagus (F); panels G and H are positive controls for EpEx staining. Original magnification ×400.From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers. PLoS ONE 5: e14130.
  • IHC-P - Anti-Human CD326 Antibody, clone MOC-31  ABD11714
    Published customer image:Mouse anti Human CD326 antibody, clone MOC-31 used for the detection of CD326 positive cells by immunohistochemistry on formalin fixed, paraffin embedded prostate tissue sections.Image caption: Immunohistochemical analysis of Ep-ICD and EpEx in prostate tissues. Panel shows (a) Nuclear/Cytoplasmic Ep-ICD immunostaining in (I) Normal, BPH, and Prostate Adenocarcinoma: Gleason Score < 7, Gleason Score = 7; and (V) Gleason Score > 7. Panel (b) Membrane EpEx immunostaining in (I) Normal BPH, and Prostate Adenocarcinoma Gleason Score < 7, Gleason Score = 7; and (V) Gleason Score > 7. Arrows show nuclear (N) Ep-ICD staining and Membrane (M) EpEx staining (Original Magnification x 400).From: Assi J, Srivastava G, Matta A, MacMillan C, Ralhan R, et al. Nuclear Ep-ICD Expression Is a Predictor of Poor Prognosis in “Low Risk” Prostate Adenocarcinomas. PLoS ONE 10(2): e0107586.
  • IHC-P - Anti-Human CD326 Antibody, clone MOC-31  ABD11714
    Published customer image:Mouse anti Human CD326 antibody, clone MOC-31 used for the detection of CD326 staining by immunohistochemistry of formalin fixed, paraffin embedded tumor tissue sections. Image caption: Immunohistochemical analysis of EpEx and Ep-ICD expression in papillary thyroid carcinomas and benign tissues.The representative photomicrographs show immunostaining of EpEx and Ep-ICD in paraffin-embedded thyroid benign nodule goiters, non-aggressive PTC and aggressive PTC tissues. Strong membranous EpEx immunostaining was observed in benign cases (A) and non-aggressive PTC tissues (C); reduced staining of membrane EpEx was observed in aggressive PTC cases (E, G). The benign thyroid nodules and non-aggressive PTC (D) showed predominant cytoplasm localization of Ep-ICD and no detectable nuclear Ep-ICD staining (B, D), while the aggressive PTC cases showed strong nuclear and cytoplasmic Ep-ICD accumulation (F, H). M, membrane staining; C, cytoplasmic staining; N, nuclear staining; Loss of M, loss of membrane expression. Original magnification × 400.From: He HC-H, Kashat L, Kak I, Kunavisarut T, Gundelach R, et al. An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma. PLoS ONE 7(9): e42893.
  • IHC-P - Anti-Human CD326 Antibody, clone MOC-31  ABD11714
    Published customer image:Mouse annti Human CD326 antibody, clone MOC-31 used for the detection of CD326 positive cells by immunohistochemistry on formailin fixed, paraffing embedded breast cancer tissue sections.Image caption:Immunohistochemical analysis of EpEx expression in breast cancer. Expression of EpEx in (I) normal breast tissues; DCIS; IDC; ILC; (V) IMC; negative control breast cancer tissue incubated with isotype specific IgG showing no detectable immunostaining for EpEX. Membranous EpEx expression was more frequently observed in breast carcinomas compared to normal tissues, except ILC (original magnification?×?400). The arrows labelled M depict membrane staining.From: Srivastava G, Assi J, Kashat L, Matta A, Chang M, Walfish PG, Ralhan R. Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients. BMC Cancer. 2014 Sep 29;14:726.
  • IHC-P - Anti-Human CD326 Antibody, clone MOC-31  ABD11714
    Published customer image:Mouse anti Human CD326 antibody, clone MOC-31 used for the detection opf CD326 positive cells in tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Immunohistochemical analysis of EpEx, Ep-ICD and ß catenin in thyroid cancer. The ATC did not show detectable membranous EpEx staining ; all the other subtypes of TC analyzed and normal thyroid tissues showed varying levels of plasma membranous EpEx staining . Nuclear Ep-ICD staining was observed in undifferentiated and poorly differentiated TC (IIA-IIC, and IIF), but not in well differentiated TC and the adjacent normal thyroid tissue (IID, IIE). Correlated with nuclear Ep-ICD staining, nuclear or cytoplasmic ß catenin staining was observed in aggressive TC (IIIA-IIIC, and IIIF), while membranous staining was observed in the less aggressive TC and the adjacent normal thyroid tissues (IIID, IIIE). Panels A, B and F- I and II, C and F III- original magnification × 40; panels A III, B III, C, D, E - I and II, D and E III- original magnification × 20. The abbreviations M, C, and N denote membranous, cytoplasmic and nuclear localization of the proteins.From: Ralhan R, Cao J, Lim T, Macmillan C, Freeman JL, Walfish PG. EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis. BMC Cancer. 2010 Jun 25;10:331.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC
Primary Accession P16422
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MOC-31
Calculated MW 34932 Da
Additional Information
Shelf Life 18 months from date of despatch.
Gene ID 4072
Other Names Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Target/Specificity Mouse anti-Human CD326 antibody, clone MOC31 recognizes human CD326, also known as Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein 314, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2 and Tumor-associated calcium signal transducer 1. Human CD326 is a 314 amino acid ~40 kDa type 1 single pass transmembrane glycoprotein containing a single thyroglobulindomain,CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium (Spizzoet al.2002).
Preservative & Stabilisers Preservative Free
Storage Storage at -20℃ only.
PrecautionsAnti-Human CD326 Antibody, clone MOC-31 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Souhami, R. L. et al. (1987) Antigens of small cell lung cancer. First International Workshop.
Lancet. 2: 325-326. 2. Ralhan R et al. (2010) Nuclear and cytoplasmic accumulation of Ep-ICD is frequently detected in human epithelial cancers.
PLoS One. 5 (11): e14130. 3. Ralhan, R. et al. (2010) EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
BMC Cancer. 10: 331. 4. Dankers, P.Y. et al. (2010) The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney.
Macromol Biosci. 10: 1345-54. 5. Kawashima, R. et al. (2011) EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.
Int J Cancer. 129: 1244-53. 6. Assi J et al. (2015) Nuclear Ep-ICD Expression Is a Predictor of Poor Prognosis in"Low Risk"Prostate Adenocarcinomas.
PLoS One. 10 (2): e0107586. 7. Kunavisarut T et al. (2012) Immunohistochemical analysis based Ep-ICD subcellular localization index (ESLI) is a novel marker for metastatic papillary thyroid microcarcinoma.
BMC Cancer. 12: 523. 8. He HC et al. (2012) An Ep-ICD based index is a marker of aggressiveness and poor prognosis in thyroid carcinoma.
PLoS One. 7 (9): e42893. 9. Srivastava G et al. (2014) Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients.
BMC Cancer. 14: 726.1. Winter, M. J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Am. J. Pathol. 163: 2139-2148.

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