|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Chicken Bursa cells.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Chicken Bu-1a, clone L22 recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development (Houssaintet al.1991).Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti-chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyartoet al.2008)|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Chicken Bu-1A Antibody, clone L22 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Pink, J.R. & Rijnbeek, A.M. (1983) Monoclonal antibodies against chicken lymphocyte surface antigens.Hybridoma. 2: 287-96. 2. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.Infect Immun. 79: 3445-54. 3. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. Eur. J. Immunol. 19: 239 - 243. 4. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.Immunogenetics. 44: 212-7. 5. Houssaint, E. et al. (1991) Early separation of B and T lymphocyte precursors in chick embryo.J Exp Med. 174: 397-406. 6. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.Poult Sci. 87: 351-5. 7. Veromaa, T. et al. (1988) Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens.Hybridoma. 7: 41-8. 8. Chaussé, A.M. et al. (2014) Susceptibility to Salmonella carrier-state: a possible Th2 response in susceptible chicks.Vet Immunol Immunopathol. 159: 16-28. 9. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.Development. 141: 3255-65. 10. Luna-Acosta, J.L. et al. (2015) Direct antiapoptotic effects of growth hormone are mediated by PI3K/AKT pathway in the chicken bursa of Fabricius.Gen Comp Endocrinol. pii: S0016-6480(15)00204-X.
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