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>   home   >   Products   >   Primary Antibodies   >   Anti-Chicken Bu-1A Antibody, clone L22 (FITC)   

Anti-Chicken Bu-1A Antibody, clone L22 (FITC)

Mouse Anti-Chicken Monoclonal Antibody

     
  • IF - Anti-Chicken Bu-1A Antibody, clone L22 (FITC) ABD11717
    Published customer image:Mouse anti Chicken Bu-1 antibody, clone L22 used for immunofluorescence studies.Image caption:F distribution of lymphoid aggregates in the MacRed chicken gut. (A-I) External views of different regions of a 1-year-old MacRed chicken showing several scattered lymphoid aggregates in the jejunum (A-C), numerous scattered lymphoid aggregates in the ileum (D-F) and a high concentration of lymphoid aggregates in the ileum Peyer's Patch. Scale bars in A-I: 500 μm. (J-L) Immunofluorescence staining of Peyers patches showing organisation of CSF1R-mApple-expressing cells (red) in relation to: (J) Bu-1+ B-cells (green), (K) TCR αβß (Vβ1)+ T-cells (green) and (L) CVI-ChNL-74.2+ macrophages (green). Scale bars in J-L: 100 μm.From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141:3255-65.
  • IF - Anti-Chicken Bu-1A Antibody, clone L22 (FITC) ABD11717
    Published customer image:Mouse anti Chicken Bu-1 antibody, clone L22 used for immunofluorescence studies.Image caption:Confocal analysis of MacRed chicken post-hatch mononuclear phagocyte populations. (A) Splenic mononuclear phagocytes and Bu-1+ B-cells from a 16-week-old MacRed chicken. Rings of transgene-expressing cells can clearly be seen surrounding the ellipsoid . (B) Bursa of Fabricius from an 8-day-old MacRed chicken: Bu-1+ B cells show arrangement of the B-cell follicles; mononuclear phagocytes are present in the medulla (M) and interfollicular region (red arrow), but not in the cortex (C) of B-cell follicles in the bursa of Fabricius. (C) Caecal tonsil B-cell follicle from a 10-week-old MacRed chicken, showing location of mononuclear phagocytes and Bu-1+ B-cells . Transgene-expressing cells concentrated in the medulla region (M) of the B-cell follicle are a dense network of FDC. (D) Microglial cells in the cerebellum of an 8-day-old MacRed chicken showing colocalisation with CD45 staining . (E) Kupffer cells showing colocalisation with CSF1R from a 13-week-old MacRed chicken liver. (F) Lung mononuclear phagocytes and Bu-1+ B-cells in the interstitial tissue of the parabronchial wall from a 16-week-old MacRed chicken. The parabronchial lumen is indicated. (G) Epidermal mononuclear phagocyte cells in epidermal sheet preparation from a 10-week-old MacRed chicken. (H) Breast muscle mononuclear phagocytes from a 16-week-old MacRed chicken co-expressing MHCII . (I) Feather pulp mononuclear phagocytes from an 8-day-old MacRed chicken co-stained with CD45 . Scale bars: 50 μm.From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141:3255-65.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Chicken
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names L22
Additional Information
Other Species Quail
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Chicken Bursa cells.
Shelf Life 12 months from date of reconstitution.
Target/Specificity Mouse anti-Chicken Bu-1a, clone L22 recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development (Houssaintet al.1991).Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti-chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyartoet al.2008)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Chicken Bu-1A Antibody, clone L22 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Pink, J.R. & Rijnbeek, A.M. (1983) Monoclonal antibodies against chicken lymphocyte surface antigens.
Hybridoma. 2: 287-96. 2. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
Infect Immun. 79: 3445-54. 3. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos.
Eur. J. Immunol. 19: 239 - 243. 4. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
Immunogenetics. 44: 212-7. 5. Houssaint, E. et al. (1991) Early separation of B and T lymphocyte precursors in chick embryo.
J Exp Med. 174: 397-406. 6. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
Poult Sci. 87: 351-5. 7. Veromaa, T. et al. (1988) Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens.
Hybridoma. 7: 41-8. 8. Chaussé, A.M. et al. (2014) Susceptibility to Salmonella carrier-state: a possible Th2 response in susceptible chicks.
Vet Immunol Immunopathol. 159: 16-28. 9. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65. 10. Luna-Acosta, J.L. et al. (2015) Direct antiapoptotic effects of growth hormone are mediated by PI3K/AKT pathway in the chicken bursa of Fabricius.
Gen Comp Endocrinol. pii: S0016-6480(15)00204-X.

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Cat# ABD11717
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