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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti-Human Beta Amyloid (aa1-17) Antibody, clone DE2B4    

Anti-Human Beta Amyloid (aa1-17) Antibody, clone DE2B4

Mouse Anti-Human Monoclonal Antibody

     
  • WB - Anti-Human Beta Amyloid (aa1-17) Antibody, clone DE2B4  ABD11718
    Published customer image:Mouse anti Human beta amyloid antibody, clone DE2B4 used for the evaluation of amyloid deposition in brain lysates by dot and western blotting.Image caption:GF-ß1 induces TPC6A? aggregation for leading to caspase activation(A) Neuroblastoma SK-N-SH cells were transiently expressed with ECFP or ECFP-TPC6A? and treated with TGF-ß1 (5 ng/ml) for indicated times, followed by fixation, permeabilization and staining with Aß antibody. The ratios of the expression of Aß to TPC6A? are shown (n = 5). (B) TPC6A aggregates (green) are present in the mitochondria of degenerative neurons in the AD hippocampus. Tissue sections were pre-stained with the Bielschowsky stain (brown to dark), and then stained with Fluoro-Jade C Red for degenerative neurons (red), specific COX4 antibody for mitochondria (cyan), DAPI for nuclei (blue) and pan-specific antibody for TPC6A (green). 600x magnification; Scale bar: 10 µm. (C) In negative controls, no primary antibody was used in the immunostaining. (D) Transiently overexpressed EYFP-TPC6A? was aggregated in SK-N-SH cells, and caspase 3 became activated (Casp3*). (E) No caspase 3 activation was observed, if ectopic TPC6A? was not aggregated. (F) TGF-ß1 increased TPC6A? aggregation and caspase 3 activation. (G–H) Wild type TPC6A , either in a native or an aggregated state, did not induce caspase 3 activation. (I–J) SK-N-SH cells were transiently overexpressed with the indicated expression constructs. The number of cells harboring protein aggregation was counted, and the extent of caspase 3 activation was measured (n = 3; Student's t tests; ~50 cells counted per experiment). (K) Colocalization of S35-phosphorylated TPC6A? with activated caspase 3 in the AD hippocampal tissue sections. A representative data is shown.From: Chang JY, Lee MH, Lin SR, Yang LY, Sun HS, Sze CI, Hong Q, Lin YS, Chou YT, Hsu LJ, Jan MS, Gong CX, Chang NS. Trafficking protein particle complex 6A delta (TRAPPC6A?) is an extracellular plaque-forming protein in the brain.Oncotarget. 2015 Feb 28;6:3578-89.
  • WB - Anti-Human Beta Amyloid (aa1-17) Antibody, clone DE2B4  ABD11718
    Published customer image:Mouse anti Human beta amyloid antibody, clone DE2B4 used for the evaluation of amyloid deposition in brain lysates by dot and western blotting.Image caption:Soluble and insoluble TPC6A aggregates are present in the hippocampi of nondemented human controls and AD samples (A) Frozen postmortem AD and control human hippocampal tissues were homogenized by sonication. After centrifugation, the insoluble pellets were further extracted and analyzed by filter retardation assay. Western blotting was then carried out using specific antibodies for TPC6A , TIAF1, Tyr33-phosphorylated WWOX (p-WWOX), NFT and Aß. No significant difference was shown in TPC6A or TIAF1 aggregation for samples between younger nondemented controls (14 samples per group; total 3 groups; 42 to 76 years old) and older AD patients (24 samples per group; total 4 groups; 71 to 90 years old). C = control; A or AD = Alzheimer's disease. (B) The supernatants of the aforementioned samples were analyzed by 7% non-reducing SDS-PAGE. Western blotting analyses show the presence of soluble aggregates of TPC6A and Aß in the representative protein preparations. Sample numbers are shown in each panel. Molecular weight markers: 170, 130, 95, 72 kDa. (C) Hippocampal tissue sections of APP/PS1-transgenic mice were stained with aliquots of TPC6A pan-specific antiserum and then with an Alexa Fluor 488-conjugated secondary antibody. Fluoro-Jade C was used for staining degenerative neurons, and nuclei stained with DAPI. TPC6A aggregates are localized in the degenerative neurons. A representative tissue section is shown. Scale bar: 50 µm. In negative controls, the sections were stained with the secondary antibody only, followed by staining with Fluoro-Jade C and DAPI. (D) Immuno-intensity was measured and normalized to negative controls (mean ± standard deviation, n = 5; **p < 0.05, student's t test).From: Chang JY, Lee MH, Lin SR, Yang LY, Sun HS, Sze CI, Hong Q, Lin YS, Chou YT, Hsu LJ, Jan MS, Gong CX, Chang NS. Trafficking protein particle complex 6A delta (TRAPPC6A?) is an extracellular plaque-forming protein in the brain.Oncotarget. 2015 Feb 28;6(6):3578-89.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, E, IP
Primary Accession P05067
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DE2B4
Calculated MW 86943 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Synthetic peptide consisting of residues 1 - 17 of the amyloid beta protein.
Shelf Life 18 months from date of despatch.
Gene ID 351
Other Names Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C99, Beta-amyloid protein 42, Beta-APP42, Beta-amyloid protein 40, Beta-APP40, C83, P3(42), P3(40), C80, Gamma-secretase C-terminal fragment 59, Amyloid intracellular domain 59, AICD-59, AID(59), Gamma-CTF(59), Gamma-secretase C-terminal fragment 57, Amyloid intracellular domain 57, AICD-57, AID(57), Gamma-CTF(57), Gamma-secretase C-terminal fragment 50, Amyloid intracellular domain 50, AICD-50, AID(50), Gamma-CTF(50), C31, APP, A4, AD1
Target/Specificity Mouse anti-Human Beta Amyloid antibody, clone DE2B4 recognizes the human beta amyloid peptide, which is the major protein component of the amyloid cores in neuritic plaques.In immunohistology the antibody detects extracellular amyloid beta with senile plaques and vessel amyloid in Alzheimer's disease brain.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Beta Amyloid (aa1-17) Antibody, clone DE2B4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Kitamoto, T. et al. (1987) Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids.
Lab. Invest. 57: 230 - 236. 2. Lee MH et al. (2010) TGF-β induces TIAF1 self-aggregation via type II receptor-independent signaling that leads to generation of amyloid β plaques in Alzheimer's disease.
Cell Death Dis. 1: e110. 3. Chang, J.Y. et al. (2012) TIAF1 self-aggregation in peritumor capsule formation, spontaneous activation of SMAD-responsive promoter in p53-deficient environment, and cell death.
Cell Death Dis. 3: e302. 4. Koronyo-Hamaoui, M. et al. (2011) Identification of amyloid plaques in retinas from Alzheimer's patients and noninvasive in vivo optical imaging of retinal plaques in a mouse model.
Neuroimage. 54 Suppl 1: S204-17. 5. Chang JY et al. (2015) Trafficking protein particle complex 6A delta (TRAPPC6A?) is an extracellular plaque-forming protein in the brain.
Oncotarget. 6 (6): 3578-89.

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