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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine CD11b Antibody, clone CC126    

Anti-Bovine CD11b Antibody, clone CC126

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti-Bovine CD11b Antibody, clone CC126  ABD11719
    Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.Image caption:CD11b surface expression and CD11b-dependent-NET formation of bovineE. bovissporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.From: Muñoz-Caro et al. Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent. Veterinary Research 2015 46:23
  • FC - Anti-Bovine CD11b Antibody, clone CC126  ABD11719
    Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD11b: FITC
  • FC - Anti-Bovine CD11b Antibody, clone CC126  ABD11719
    Staining of bovine peripheral blood monocytes with Mouse anti Bovine CD11b followed by Goat anti Mouse IgG:FITC
  • FC - Anti-Bovine CD11b Antibody, clone CC126  ABD11719
    Published customer image:Mouse anti Bovine CD11b antibody, clone CC126 used for the detection of CD11b on ovine leukocytes by flow cytometryImage caption:Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 Feb 23;42:37.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names CC126
Additional Information
Other Species Ga,Sh
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine CD11b Antibody, clone CC126 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. 2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. 3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. 4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytesand modulate CD18 expression.
Vet Res. 39: 50. 5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. 6. Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. 7. Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.
J Dairy Res. 78 (1): 43-8. 8. Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.
J Leukoc Biol. 82: 603-7. 9. Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.
J Virol. 85: 12982-94. 10. Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.
J Dairy Sci. 95: 3877-87. 11. Muñoz-Caro T et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Vet Res. 46: 23. 12. Dedieu L et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.
Scand J Immunol. 62 (6): 528-38. 13. Flameng W et al. (2014) Coating with fibronectin and stromal cell-derived factor-1a of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2. 14. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405 (1): 110-9. 15. McClenahan DJ et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.
Am J Vet Res. 61 (4): 380-6. 16. Foulon E & Foucras G (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. 17. Lin Y et al. (2013) Spatial distribution of polychlorinated naphthalenes in the atmosphere across North China based on gridded field observations.
Environ Pollut. 180: 27-33. 18. Lamote I et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.
Vet Res. 37 (1): 61-74. 19. Weber B et al. (2013) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.
J Tissue Eng Regen Med. Jul 24. [Epub ahead of print] 20. Reber AJ et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.
Vet Immunol Immunopathol. 123 (3-4): 186-96. 21. Ozawa T et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36 (1): 21-7. 22. Zoldan K et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. 23. Fach SJ et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.
Vet Immunol Immunopathol. 112 (3-4): 171-82. 24. Godoy RF et al. (2014) Do progenitor cells from different tissue have the same phenotype?
Res Vet Sci. 96 (3): 454-9. 25. Fach SJ et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
Viral Immunol. 20 (1): 119-30. 26. Sassa Y et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.
Vet Immunol Immunopathol. 133 (1): 33-9. 27. Summers C et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 106 (3-4): 285-94. 28. Pilla R et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.
J Dairy Sci. 96 (3): 1653-60.

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Cat# ABD11719
(40 western blots)
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