|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine CD11b Antibody, clone CC126 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47. 2. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet Immunol Immunopathol. 39 (1-3): 283-90. 3. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32: 837-49. 4. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytesand modulate CD18 expression. Vet Res. 39: 50. 5. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.Vet Res. 42: 37. 6. Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. 7. Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.J Dairy Res. 78 (1): 43-8. 8. Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.J Leukoc Biol. 82: 603-7. 9. Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.J Virol. 85: 12982-94. 10. Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.J Dairy Sci. 95: 3877-87. 11. Muñoz-Caro T et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.Vet Res. 46: 23. 12. Dedieu L et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.Scand J Immunol. 62 (6): 528-38. 13. Flameng W et al. (2014) Coating with fibronectin and stromal cell-derived factor-1a of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2. 14. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.Virology. 405 (1): 110-9. 15. McClenahan DJ et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.Am J Vet Res. 61 (4): 380-6. 16. Foulon E & Foucras G (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.J Immunol Methods. 339 (1): 1-10. 17. Lin Y et al. (2013) Spatial distribution of polychlorinated naphthalenes in the atmosphere across North China based on gridded field observations.Environ Pollut. 180: 27-33. 18. Lamote I et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.Vet Res. 37 (1): 61-74. 19. Weber B et al. (2013) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.J Tissue Eng Regen Med. Jul 24. [Epub ahead of print] 20. Reber AJ et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.Vet Immunol Immunopathol. 123 (3-4): 186-96. 21. Ozawa T et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.Vet Res Commun. 36 (1): 21-7. 22. Zoldan K et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.Dev Comp Immunol. 47 (2): 254-63. 23. Fach SJ et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.Vet Immunol Immunopathol. 112 (3-4): 171-82. 24. Godoy RF et al. (2014) Do progenitor cells from different tissue have the same phenotype?Res Vet Sci. 96 (3): 454-9. 25. Fach SJ et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.Viral Immunol. 20 (1): 119-30. 26. Sassa Y et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.Vet Immunol Immunopathol. 133 (1): 33-9. 27. Summers C et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.Vet Immunol Immunopathol. 106 (3-4): 285-94. 28. Pilla R et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.J Dairy Sci. 96 (3): 1653-60.
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