|Application ||IHC-P, IHC-F, FC, IP, IH|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||Porcine peripheral blood lymphocytes|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD45 Allotypic Variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as Leucocyte common antigen (LCA). The antibody has been shown to recognise pigs of strains Large White and German Landrace. Another antibody K252.1E4, recognizes a monomorphic determinant on porcine CD45. The latter, used in conjunction with MAC323 it is noted that MCA323 stains a subset of antimals that stain with K252.1E4 indicating loss of the epitope in MCA323+ve animals rather than the loss of CD45 anigen. Cross-breeding studies in positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelialn autosomaly dominant pattern (Binns et al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD45 Allotypic Variant Antibody, clone MAC323 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig. Immunology 86: 25-33. 2. Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.Cardiovasc Revasc Med. 7: 136-45. 3. Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.Pediatr Surg Int. 19: 326-30. 5. Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.J Clin Invest. 113: 1607-14.4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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