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Anti-Human Aggrecan Antibody, clone 7D4

Mouse Anti-Human Monoclonal Antibody

  • E - Anti-Human Aggrecan Antibody, clone 7D4  ABD11724
    Recombinant human aggrecan detected with Mouse anti Human Aggrecan followed by Goat anti Mouse IgG:Dylight®800. Visualised using a LI-COR Odyssey® infrared imaging system
  • IHC-P - Anti-Human Aggrecan Antibody, clone 7D4  ABD11724
    Published customer image:Mouse anti Human aggrecan antibody, clone 7D4 used for the detection of aggrecan in bovine synovial explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sectionsImage caption:nfluence of TGF-ß1 on the BMP-2-induced deposition of cartilaginous proteins in synovial explants (immunohistochemical analysis). Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan (2nd row), type-II collagen (3rd row), type-IX collagen (4th row), type-X collagen (5th row) or COMP (6th row). BMP-2 was applied at a concentration of 200 ng/ml, and TGF-β1 at one of 10 ng/ml. Each agent was introduced at the onset of the 6-week culturing period and was present throughout its course. Native metacarpal-joint cartilage (final vertical column) served as a positive control. Cell nuclei (2nd to 6th rows) were counterstained with haematoxylin. All photomicrographs are represented at the same final magnification. Bar?=?100 μm.From: Shintani N, Siebenrock KA, Hunziker EB TGF-β1 Enhances the BMP-2-Induced Chondrogenesis of Bovine Synovial Explants and Arrests Downstream Differentiation at an Early Stage of Hypertrophy.PLoS ONE 8(1): e53086.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16112
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 7D4
Calculated MW 250193 Da
Additional Information
Other Species B
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Purified human articular cartilage aggrecan
Shelf Life 18 months from date of despatch.
Other Names Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2, ACAN, AGC1, CSPG1, MSK16
Target/Specificity Mouse anti-Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintinoet al.2009).  Mouse anti-Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, that has been identified as a cluster 1 isoform.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Aggrecan Antibody, clone 7D4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebral cortex.
J Cell Mol Med. 13 (9B): 3151-73. 2. Shintani, N. and Hunziker, E.B. (2011) Differential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: Influence of microenvironment, tissue origin and growth factor.
Eur Cell Mater. 22: 302-20. 3. Gaál B et al. (2014) Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
Neuroscience. 258: 162-73. 4. Suttkus, A. et al. (2014) Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
Cell Death Dis. 5: e1119. 5. Betre, H. et al. (2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering
Engineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30. 6. Shintani N et al. (2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.
PLoS One. 8 (1): e53086. 7. Murakami, T. & Ohtsuka, A. (2003) Perisynaptic barrier of proteoglycans in the mature brain and spinal cord.
Arch Histol Cytol. 66 (3): 195-207.

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Cat# ABD11724
(40 western blots)
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