|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared from ascites|
|Immunogen||Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, CD3e, CD3E|
|Target/Specificity||Mouse anti-Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen, a complex of at least three polypeptides of 20, 19 and 17kDa expressed by T cells (Chenet al.1986). The antibody also co-precipitates a 90kDa heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of 40kDa and 50 kD.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Chicken CD3 Antibody, clone CT-3 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Chen, C. L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.J. Exp. Med. 164: 375-380. 2. Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytesVet Immunol Immunopathol. 77: 121 - 132 3. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.Infect Immun. 79: 3445-54. 4. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.Development. 141: 3255-65.
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