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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human HLA E Antibody, clone MEM-E/02    

Anti-Human HLA E Antibody, clone MEM-E/02

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti-Human HLA E Antibody, clone MEM-E/02  ABD11737
    Western blot analysis of A431 human epidermoid carcinoma whole cell lysate probed with Mouse anti Human HLA E antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13747
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-E/02
Calculated MW 40157 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from ascites
Immunogen Recombinant HLA-E.
Shelf Life 18 months from date of despatch.
Gene ID 3133
Other Names HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E, HLA-E, HLA-6.2, HLAE
Target/Specificity Mouse anti-Human HLA-E antibody, clone MEM-E/02 reacts with the denatured heavy chain of the non-classical MHC class I HLA-E molecule. HLA-E exists as a heterodimer consisting of a heavy chain and a light chain, beta-2-microglobulin.Mouse anti-Human HLA-E antibody, clone MEM-E/02 does not recognize native HLA-E by flow cytometry and does not cross react with HLA-A, -B, -C or G.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human HLA E Antibody, clone MEM-E/02 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Cui, C.H. et al. (2011) Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy.
Hum Mol Genet. 20 (2): 235-44. 2. Derré, L. et al. (2006) Expression and release of HLA-E by melanoma cells and melanocytes: potential impact on the response of cytotoxic effector cells.
J Immunol. 177: 3100-7. 3. Menier; C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64: 315-26. 4. Coupel, S. et al. (2007) Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
Blood. 109: 2806-14. 5. Ashrafi, G.H. et al. (2005) E5 protein of human papillomavirus type 16 selectively downregulates surface HLA class I.
Int J Cancer. 113: 276-83. 6. Trichet, V. et al. (2006) Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2 T cells revealed by target cell beta2-microglobulin knockdown.
J Immunol. 177: 6129-36. 7. Griffin, C. et al. (2005) Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18.
J Gen Virol. 86: 2999-3008. 8. Djajadiningrat, R.S. et al. (2015) Classic and nonclassic HLA class I expression in penile cancer and relation to HPV status and clinical outcome.
J Urol. 193 (4): 1245-51.

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Cat# ABD11737
(40 western blots)
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