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>   home   >   Products   >   Primary Antibodies   >   Anti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE)   

Anti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE)

Mouse Anti-Sheep Monoclonal Antibody

     
  • FC - Anti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE) ABD11744
    Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:FITC
  • FC - Anti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE) ABD11744
    Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR:RPE
  • IF - Anti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE) ABD11744
    Published customer image:Phycoerythrin conjugated Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 used for the identification of MHC class II expressing cells in bovine nasopharyngeal tissue sections by immunofluorescence.Image caption:Immunofluorescent detection of persistent FMDV in nasopharyngeal mucosa.Dorsal nasopharynx, steer #626, 37dpe, FMDV O1 Manisa. Immunomicroscopy, A) Low magnification view of an epithelial invagination with a focal cluster of FMDV-antigen-positive cells within superficial epithelial surface, scale bar 50 µm. B-F) Higher magnification views of region of interest identified in A (dashed box). Cells containing FMDV-VP1 are in the superficial epithelium and are cytokeratin-positive. Few MHC-II and CD11c positive cells are present within and below epithelium, but do not contain FMDV-VP1. Indirect fluorescence technique with differential interference contrast, antibody labels color-coded in bottom panel, asterisks indicate channels present in each panel, scale bar 25 µm.From: Pacheco JM, Smoliga GR, O’Donnell V, Brito BP, Stenfeldt C, Rodriguez LL, et al. Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression. PLoS ONE 10(5): e0125698.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 28.1
Additional Information
Other Species B,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Ovine alveolar macrophages.
Shelf Life 12 months from date of reconstitution.
Target/Specificity Mouse anti-Sheep MHC Class II DQ DR antibody, clone 28.1 recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules, which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain. Mouse anti-Sheep MHC Class II DQ DR antibody, clone 28.1 recognises ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. (Ballingall, K. et al. 1995).
Preservative & Stabilisers 0.09% Sodium azide; Stabilising agent
Storage Storage at +4ºC. DO NOT FREEZE.
PrecautionsAnti-Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 (RPE) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Puri, N. et al. (1985) Sheep lymphocyte antigens (OLA) II. Major histocompatibility complex class II molecules.
Immunology. 56: 725 - 733. 2. Puri, N. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet. Immunol. Immunopathol. 15: 59 - 86. 3. Puri, N. et al. (1987) Sheep MHC class II molecules. I. Immunochemical characterization.
Immunology. 62: 567 - 573. 4. Puri, N. et al. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecule.
Immunology. 62: 575 - 580. 5. Puri, N. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
Hum. Immunol. 20: 195 - 207. 6. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. 7. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS One. 7: e30430. 7. Ballingall. K. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II - Specific monoclonal antibodies using mouse L - Cell transfectants.
Anim. Genet. 26: 79-84.

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Cat# ABD11744
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