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Anti-Dengue Virus Antibody, clone Dengue 1-11(3)

Mouse Anti-Viral Monoclonal Antibody

  • E - Anti-Dengue Virus Antibody, clone Dengue 1-11(3)  ABD11751
    Published customer image:Mouse anti-Dengue virus antibody, clone Dengue 1-11 used for the evaluation of dengue viral load by capture ELISA.Image caption:Anti-DIII sera recognise viral E protein. (A) Immunofluorescence of Vero cells infected with DENV of each serotype, reacted with the serotype-specific anti-DIII pools of sera (top row), the negative control sera (ctrl., middle row) and mAb 4G2 (bottom row). In each case, non-infected cells (n.i., rightmost column) were also used as controls. Bars represent 50 µm. (B) ELISA on whole infective viral particles. Anti-DIII serotype-specific pools diluted to 100 ng/ml and sera from mock-immunised animals were used onto virus particles captured on plates coated with a human serum reactive against all four serotypes. mAb Dengue 1–11 reactive against DENV1 E (at 1 µg/ml) and a Dengue pan-reactive serum against all four serotypes were used as positive controls.From: Poggianella M, Slon Campos JL, Chan KR, Tan HC, Bestagno M, Ooi EE, et al. Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.PLoS Negl Trop Dis 9: e0003947.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Virus
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names Dengue 1-11(3)
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Dengue virus antibody, clone Dengue 1-11 (3) recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus.Clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4. The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Dengue Virus Antibody, clone Dengue 1-11(3) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Gubler, D.J. (1998) Dengue and Dengue Hemorrhagic Fever.
Clin. Micro. Rev. 11: 480-496. 2. Philip Samuel,P. and Tyagi, B.K. (2006) Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes.
Indian J. Med. Res. 123: 612-628. 3. Lorono-Pino, M.A. et al. (1999) Common occurrence of concurrent infections by multiple dengue virus serotypes.
Am. J. Trop. Med. Hyg. 61: 725-730. 4. Kim,T.G. et al. (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.
Protein Expr Purif. 74:236-41. 5. Milic NL et al. (2015) Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus).
Dev Comp Immunol. pii: S0145-305X(15)00044-0. 6. Poggianella, M. et al. (2015) Dengue E Protein Domain III-Based DNA Immunisation Induces Strong Antibody Responses to All Four Viral Serotypes.
PLoS Negl Trop Dis. 9 (7): e0003947.

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Cat# ABD11751
(40 western blots)
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