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Anti-Mouse CD70 Antibody, clone FR70 (FITC)

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD70 Antibody, clone FR70 (FITC) ABD11753
    Staining of LPS activated mouse spleen cellswith Rat anti Mouse CD70: Alexa Fluor® 488
  • FC - Anti-Mouse CD70 Antibody, clone FR70 (FITC) ABD11753
    Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70: Alexa Fluor® 647
  • FC - Anti-Mouse CD70 Antibody, clone FR70 (FITC) ABD11753
    Staining of LPS stimulated mouse spleen cells with rat anti-mouse CD70 : Biotin
  • FC - Anti-Mouse CD70 Antibody, clone FR70 (FITC) ABD11753
    Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O55237
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names FR70
Calculated MW 21919 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen A20 mouse B cell lymphoma cell line
Shelf Life 18 months from date of despatch.
Gene ID 21948
Other Names CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70, Cd70, Cd27l, Cd27lg, Tnfsf7
Target/Specificity Rat anti-Mouse CD70 antibody, clone FR70 recognizes mouse CD70, a 30-33kD type II transmembrane protein belonging to the TNF superfamily. CD70 is not expressed on resting T and B lymphocytes but expression is induced on activation. Interaction of CD70 with its ligand CD27 is reported to play an important role in T-cell co-stimulation and the regulation of T-dependant B-cell activation and antibody production.Clone FR70 is reported to block the functions of CD70 and has been used to block the interactions between CD27 and CD70 (Oshimaet al.).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Mouse CD70 Antibody, clone FR70 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Oshima, H. et al. (1998) Characterization of murine CD70 by molecular cloning and mAb.
Int. Immunol.10:517 – 526. 2. Takeda, K. et al. (2000) CD27-Mediated Activation of Murine NK Cells.
J. Immunol.164:1741-1745. 3. Akiba, H. et al. (2000) Critical Contribution of OX40 Ligand to T Helper Cell Type 2 Differentiation in Experimental Leishmaniasis.
J. Exp. Med.191:375-380. 4. Akiba, H. et al. (1999) CD28-Independent Costimulation of T Cells by OX40 Ligand and CD70 on Activated B Cells.
J. Immunol.162:7058 – 7066. 5. Hadziahmetovic, M. et al. (2012) Microarray Analysis of Murine Retinal Light Damage Reveals Changes in Iron Regulatory, Complement, and Antioxidant Genes in the Neurosensory Retina and Isolated RPE.
Invest Ophthalmol Vis Sci. 53: 5231-41.

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Cat# ABD11753
(40 western blots)
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