|Application ||WB, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||ConA/PMA activated porcine peripheral blood cells.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD5, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmulleret al.1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55kDa + 60kDa doublet as the control antibody (Pescowitzet al.1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmulleret al.1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+).No reactivity of Mouse anti-pig CD5 was noted with canine, bovine, equine or human tissue samples (Doménechet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD5 Antibody, clone 1H6/8 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Pescovitz, M. D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD5.Vet. Immunol. Immunopathol. 60: 269-273 2. Saalmuller, A. et al. (1998) Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens.Vet. Immunol. Immunopathol. 60: 207-228 3. Domenech, N. et al. (2003) A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.Hybrid. Hybridomics. 22:179-182 4. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.Arch. Virol. 148: 2307-2323.5. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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