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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig CD5 Antibody, clone 1H6/8 (FITC)   

Anti-Pig CD5 Antibody, clone 1H6/8 (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig CD5 Antibody, clone 1H6/8 (FITC) ABD11754
    Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD5: FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 1H6/8
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen ConA/PMA activated porcine peripheral blood cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig CD5, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmulleret al.1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55kDa + 60kDa doublet as the control antibody (Pescowitzet al.1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine γ/δ T cells (Saalmulleret al.1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+).No reactivity of Mouse anti-pig CD5 was noted with canine, bovine, equine or human tissue samples (Doménechet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig CD5 Antibody, clone 1H6/8 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Pescovitz, M. D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD5.
Vet. Immunol. Immunopathol. 60: 269-273 2. Saalmuller, A. et al. (1998) Overview of the Second International Workshop to define swine cluster of differentiation (CD) antigens.
Vet. Immunol. Immunopathol. 60: 207-228 3. Domenech, N. et al. (2003) A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
Hybrid. Hybridomics. 22:179-182 4. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection.
Arch. Virol. 148: 2307-2323.5. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD11754
(40 western blots)
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