|Application ||WB, IP|
|Calculated MW||18119 Da|
|Purification||Ig fraction prepared by ammonium sulphate precipitation|
|Immunogen||Human p21 protein|
|Shelf Life||18 months from date of despatch.|
|Other Names||Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1|
|Target/Specificity||ABD11758 recognises the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1.The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.ABD11758 detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human p21/WAF1 Antibody, clone WA-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Horky, M. et al. (1997) nduction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy.Physiol. Res. 46: 233-235. 2. Kotala, V. et al. (2001) Potent induction of wild-type p53-dependent transcription in tumour cells by a synthetic inhibitor of cyclin-dependent kinases.Cell. Mol. Life Sci. 58: 1333-1339. 3. Kolar, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer.Mol. Pathol. 53: 15-18. 4. Khanna, S.J. et al. (2001) V-Abl protein-tyrosine kinase up-regulates p-21 WAF-1 in cell cycle arrested and proliferating myeloid cells.J. Biol. Chem. 276: 11143-11150. 5. Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.EMBO J. 21: 6771-6780. 6. Blaydes, J.P. et al. (2001) Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription.J Biol Chem. 276: 4699-708.
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