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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Bovine TNF alpha Antibody, clone CC328 (Biotin)   

Anti-Bovine TNF alpha Antibody, clone CC328 (Biotin)

Mouse Anti-Bovine Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06599
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CC328
Calculated MW 25567 Da
Additional Information
Other Species Sh
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant bovine TNF alpha
Shelf Life 18 months from date of despatch.
Gene ID 280943
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity Mouse anti-Bovine TNF alpha antibody, clone CC328 recognizes bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine TNF alpha Antibody, clone CC328 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.
Vet. Immunol. Immunopathol. 95: 21-31. 2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.
Infect Immun. 71 (11): 6420-5. 3. Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells.
Cell Microbiol. 5 (10): 709-16. 4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.
Vet Immunol Immunopathol. 135 (3-4): 320-4. 5. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. 6. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis.
Vet Res. 42: 49. 7. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.

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Cat# ABD11759
(40 western blots)
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