|Application ||WB, IF, FC, E|
|Calculated MW||40524 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide corresponding to the C-Terminal region of non-Phosphorylated human CD195 conjugated to BSA.|
|Shelf Life||18 months from date of despatch.|
|Other Names||C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195, CCR5, CMKBR5|
|Target/Specificity||Mouse anti-Human CD195 (Non-Phosphorylated) antibody, clone R-C10 recognizes human CD195, also known as CCR5 in it’s non-phosphorylated form (Pollok-Koppet al.2003). Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes.CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation, phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases (Pollok-Koppet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD195 (Non-Phosphorylated) Antibody, clone R-C10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Pollok-Kopp, B. et al. (2003) Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies.J Biol Chem. 278 (4): 2190-8. 2. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.J Biol Chem. 280 (45): 37503-15.
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