|Application ||WB, IF, FC, E|
|Calculated MW||40524 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide corresponding to the C-Terminal region of non-Phosphorylated human CD195 conjugated to BSA.|
|Shelf Life||18 months from date of despatch.|
|Other Names||C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195, CCR5, CMKBR5|
|Target/Specificity||Mouse anti-Human CD195 (Non-Phosphorylated) antibody, clone R-C10 recognizes human CD195, also known as CCR5 in it’s non-phosphorylated form (Pollok-Koppet al.2003). Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes.CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation, phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases (Pollok-Koppet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD195 (Non-Phosphorylated) Antibody, clone R-C10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Pollok-Kopp, B. et al. (2003) Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies.J Biol Chem. 278 (4): 2190-8. 2. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.J Biol Chem. 280 (45): 37503-15.
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