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Anti-Human CD195 (pSer337) Antibody, clone V14/2

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD195 (pSer337) Antibody, clone V14/2  ABD11762
    Staining of permeablised RBL-CCR5 transfectants following stimulation with PMA, with Mouse anti Human CD195 (PHOSPHORYLATED AT SER337)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51681
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names V14/2
Calculated MW 40524 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Synthetic phospho peptide corresponding to the C-Terminal region of human CD195 conjugated to BSA.
Shelf Life 18 months from date of despatch.
Gene ID 1234
Other Names C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195, CCR5, CMKBR5
Target/Specificity Mouse anti-Human CD195 (pSer337) antibody, clone V14/2 recognizes human CD195 when phosphorylated at Ser 337. CD195 is a cell surface antigen of ~40 kDa, also known as CCR5 (Pollok-Koppet al.2003). Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes. Following ligand stimulation, CD195 is rapidly phosphorylated within the C-terminal domain. Mouse anti-Human CD195 (pSer337) antibody, clone V14/2 recognizes a band of ~40kDa on western blots of RBL-CCR5 cell lysates.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD195 (pSer337) Antibody, clone V14/2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Pollok-Kopp, B. et al. (2003) Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies.
J Biol Chem. 278 (4): 2190-8. 2. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280 (45): 37503-15.

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Cat# ABD11762
(40 western blots)
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