|Application ||IF, FC|
|Calculated MW||43195 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||CTLL cells transfected with mouse CD123.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, Interleukin-3 receptor class II alpha chain, CD123, Il3ra, Sut-1|
|Target/Specificity||Rat anti-Mouse CD123 antibody, clone 5B11 recognizes murine Interleukin-3 receptor subunit alpha, also known as CD123. CD123 is a 396 amino acid ~ 70 kDa single pass, type 1 transmembrane glycoprotein expressed on haematopoietic progenitors and most myeloid cells including basophils and mast cells. Certain mouse strains including A/J, AKR/J and NZB/B1NJ are reported to have low levels of CD123 expression due to aberrant splicing of the IL-3R alpha subunit gene.In mouse, CD123 can associate with either the common beta subunit CDw131 or with the murine IL-3 beta subunit to form a high affinity receptor for IL-3. IL-3 can bind to CD123 itself but with low affinity. Binding of IL-3 to the IL-3 receptor complex induces the proliferation and differentiation of haematopoietic cells.Rat anti-Mouse CD123 antibody, clone 5B11 does not block the binding of IL-3 to the high affinity IL-3 receptor.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD123 Antibody, clone 5B11 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Ichihara, M. et al. (1995) Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene.EMBO J. 14 (5): 939-50. 2. Xu, W. et al. (2008) Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. J Immunol. 181:276-87. 3. O'Farrell, AM. et al. (1998) Signaling pathways activated in a unique mast cell line where interleukin-3 supports survival and stem cell factor is required for a proliferative response.Blood. 87:3655-68.
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