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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Pig TNF alpha Antibody, clone 6E10    

Anti-Pig TNF alpha Antibody, clone 6E10

Mouse Anti-Pig Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, Func
Primary Accession P23563
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 6E10
Calculated MW 25254 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant porcine TNF alpha
Shelf Life 18 months from date of despatch.
Gene ID 397086
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity ABD11765 is specific for porcine tumour necrosis factor alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells, see Su, X. et al. for details.
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Pig TNF alpha Antibody, clone 6E10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Su, X. et al. (1992) Monoclonal antibodies to porcine tumor necrosis factor alpha development of an enzyme-linked immunoabsorbant assay.
J. Immunoassay 13:253-272. 2. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29. 3. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78.

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Cat# ABD11765
(40 western blots)
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