|Application ||WB, E, Func|
|Calculated MW||25254 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant porcine TNF alpha|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||ABD11765 is specific for porcine tumour necrosis factor alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells, see Su, X. et al. for details.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Pig TNF alpha Antibody, clone 6E10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Su, X. et al. (1992) Monoclonal antibodies to porcine tumor necrosis factor alpha development of an enzyme-linked immunoabsorbant assay.J. Immunoassay 13:253-272. 2. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. J Immunol Methods. 327: 18-29. 3. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.Vet Immunol Immunopathol. 124: 367-78.
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