|Calculated MW||15119 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant bovine IL-4.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1, IL4|
|Target/Specificity||Mouse anti-Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussleret al.1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti-Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabelet al.2011) and ovine samples (Olivieret al.2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaroet al.2012).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Interleukin-4 Antibody, clone CC313 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.J Immunol Methods. 301 (1-2): 114-23. 2. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.J Immunol. 174 (11): 6702-15. 3. Sipka, A. et al. (2013) Prednisolone and cefapirin act synergistically in resolving experimental Escherichia colimastitisJ Dairy Sci. 96: 4406-18. 4. Stabel, J.R. et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasiiandM. bovis.Clin Vaccine Immunol. 20: 848-57. 5. Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium aviumsubsp.paratuberculosisvaccine.Clin Vaccine Immunol. 18: 2079-89. 6. Dacal, V. et al. (2009) Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).Vet Immunol Immunopathol. 131: 59-64. 7. Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.PLoS One. 7: e30430. 8. Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.Res Vet Sci. 93: 662-7. 9. Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.Parasite Immunol. 31: 72-7. 10. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
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