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>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine Interleukin-4 Antibody, clone CC313    

Anti-Bovine Interleukin-4 Antibody, clone CC313

Mouse Anti-Bovine Monoclonal Antibody

  • E - Anti-Bovine Interleukin-4 Antibody, clone CC313  ABD11768
    Published customer image:Mouse anti Bovine Interleukin-4 antibody, clone CC313 used as a capture reagent for the detection of interleukin-4 in supernatants of cultured bovine cells using a sandwich ELIDSA together with biotinylated clone CC314 as a detection reagent.Image caption:CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 105 allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [3H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).From: Olivier M, Foret B, Le Vern Y, Guilloteau LA Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.PLoS ONE 7: e30430.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30367
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CC313
Calculated MW 15119 Da
Additional Information
Other Species Sh,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant bovine IL-4.
Shelf Life 18 months from date of despatch.
Gene ID 280824
Other Names Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1, IL4
Target/Specificity Mouse anti-Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussleret al.1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti-Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabelet al.2011) and ovine samples (Olivieret al.2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaroet al.2012).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Interleukin-4 Antibody, clone CC313 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.
J Immunol Methods. 301 (1-2): 114-23. 2. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174 (11): 6702-15. 3. Sipka, A. et al. (2013) Prednisolone and cefapirin act synergistically in resolving experimental Escherichia colimastitis
J Dairy Sci. 96: 4406-18. 4. Stabel, J.R. et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasiiandM. bovis.
Clin Vaccine Immunol. 20: 848-57. 5. Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium aviumsubsp.paratuberculosisvaccine.
Clin Vaccine Immunol. 18: 2079-89. 6. Dacal, V. et al. (2009) Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).
Vet Immunol Immunopathol. 131: 59-64. 7. Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430. 8. Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
Res Vet Sci. 93: 662-7. 9. Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
Parasite Immunol. 31: 72-7. 10. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.

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Cat# ABD11768
(40 western blots)
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