|Application ||WB, IHC-F, FC, IP|
|Calculated MW||58001 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Synthetic peptide corresponding to amino acids 22-36 of human lck.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tyrosine-protein kinase Lck, 188.8.131.52, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK, LCK|
|Target/Specificity||Mouse anti-Human Lck antibody, clone Lck-01 recognizes Tyrosine-protein kinase Lck, also known as Lck, p56lck, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck or T cell-specific protein-tyrosine kinase. Lck is a 508 amino acid ~56 kDa cytosolic non-receptor protein tyrosine kinase, primarily expressed in T lymphocytes and natural killer cells.Lck is a pivotal enzyme for normal T cell development and TCR-mediated signal transduction. Lck is physically associated with the cytoplasmic region of T cell specific molecules CD4 and CD8 (Bolen & Veillette 1989) and plays an important role in the phosphorylation of several proteins further downstream in the T cell signalling cascade (Veilletteet al.1988).Mutations of the Lck gene can lead to immunodeficiency 22 (IMD22), a condition characterized by T-cell dysfunction, patients presenting with recurrent infections, diarrhea and general malaise (Goldmanet al.1998).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Lck Antibody, clone LCK-01 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Romagnoli, P et al. (2001) A potential role for protein tyrosine kinase p56lck in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness.Int. Immunol. 13(3):305-312. 2. Cebecauer, M. et al. (1998) Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.Biochem. Biophys. Res. Commun. 243(3):706-710. 3. Kumar, S. et al. (2011) Disruption of HLA-DR raft, deregulations of Lck-ZAP-70-Cbl-b cross-talk and miR181a towards T cell hyporesponsiveness in leprosy.Mol Immunol. 48 (9-10): 1178-90.
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