|Calculated MW||46981 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Recombinant intracellular fragment (amino acids 97-432) of human Cbp/PAG|
|Shelf Life||18 months from date of despatch.|
|Other Names||Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Csk-binding protein, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp, PAG1, CBP, PAG|
|Target/Specificity||Mouse anti-Human PAG antibody, clone MEM-255 recognizes humanr Phosphoprotein Associated with Glycosphingolipid-enriched microdomains (PAG), also known as csk-binding protein (Cbp) or Transmembrane phosphoprotein Cbp. PAG is a single pass type II transmembrane protein. Mouse anti-Human PAG antibody, clone MEM-255 recognizes an epitope (aa 235-280) located in the cytoplasmic domain which localizes to membrane lipid rafts and is involved in regulation of T-Cell activation.The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs).Evidence has shown that by binding to the SH2 domain of the PTK csk, a major negative regulator of src-family kinases, PAG acts not only acts as a ‘co-factor’ for relocating csk to substrate rich microdomains, but also increases the affinity of csk for src-family kinases, through the formation of PAG-csk complexes.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human PAG Antibody, clone MEM-255 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Brdickova, N et al. (2001) Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton.FEBS Lett. 507: 133-136.1. Brdicka, T. et al. (2000) Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase Csk and is involved in regulation of T cell activation.J. Exp. Med. 191(9): 1591-1604.2. Ohtake, H. et al. (2002) Cutting Edge: Transmembrane phosphoprotein Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains as a negative feedback regulator of mast cell signaling through the FcepsilonRI.J. Immunol. 168: 2087-2090.
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