|Application ||WB, IF, IP|
|Calculated MW||58574 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant fragment of human Lyn (amino acids 8-238)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tyrosine-protein kinase Lyn, 188.8.131.52, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8|
|Target/Specificity||Mouse anti-Human lyn antibody, clone LYN01 recognizes Tyrosine-protein kinase Lyn, also known as p56lyn, p53lyn, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog or Lck/Yes-related novel protein tyrosine kinase a 512 amino acid, ~56kDa member of the src family of non-receptor protein tyrosine kinases (PTKs), predominantly expressed in tissues of haematopoietic origin.The interaction between lyn and syk, is crucial for regulating the response of both mast cells and basophils, following Fc epsilon R1 receptor aggregation and activation. Once activated, the rapid tyrosine phosphorylation of ITAMs by lyn, serves as a binding site for the recruitment and activation of syk, triggering the phosphorylation of further adaptor and signaling molecules further down a complex signaling cascade. This chain of events involving receptor aggregation, activation and tyrosine phosphorylation of ITAMs, is also vital for the survival and differentiation of B cells through the interaction of lyn and syk with the B cell receptor (BCR).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Lyn Antibody, clone LYN01 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Amoui, M. et al. (1997) Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors.Eur. J. Immunol. 27: 321-328. 2. Draberova, L. et al. (1996) Thy-1 mediated activation of rat mast cells: the role of Thy-1 membrane microdomains.Immunology 87:141-148.1. Kovarova, M. et al. (2001) Structure-function analysis of lyn kinase association with lipid rafts and initiation of early signaling events after Fc epsilon receptor I aggregation.Mol. Cell Biol. 21(24): 8318-8328.
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