Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11    

Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11

Mouse Anti-Sheep Monoclonal Antibody

     
  • IHC - Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11  ABD11789
    Published investigator image:Mouse anti Sheep CD230 (PrPSc) antibody, clone 2G11 used for the detection of prion protein in scrapie infected sheep by immunohistohemistry.Image caption:Photomicrographs of retinal cross-sections of unaffected (a, d) and naturally scrapie-affected (b, c, e) sheep. All layers of the control retina show normal organization and cytoarchitecture (a). Ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor segments (PS) and choroid (CHOR). Bar: 100 µm. In the scrapie-affected retina, not only was there a disorganization and thickening of the PS layer, but also an overall decrease of cellular density within the INL and ONL (b). Arrowhead indicates displaced photoreceptor nuclei in the PS layer. Bar: 100 µm. Immnohistochemical detection of abnormal PrP deposits (peroxydase-DAB, brown deposits-2G11 monoclonal mouse antibody) in a scrapie retina (c). Asterisks indicate the amorphous material accumulated in the photoreceptor outer segment layer. Bar: 100 µm. Müller cells labelling (GFAP rabbit polyclonal antibody peroxydase-DAB, brown coloration) in the retina from a control (d) and a naturally-scrapie affected sheep (e). Bars: 300 µm.From: Regnier A, Andreoletti O, Albaric O, Gruson DC, Schelcher F, Toutain PL. Clinical, electroretinographic and histomorphometric evaluation of the retina in sheep with natural scrapie. BMC Vet Res. 2011 Jun 6;7:25.
  • IHC-P - Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11  ABD11789
    Published customer image:Mouse anti sheep CD230 antibody, clone 2G11 used for the detection of prion protein in ovine brain by immunohistochemistry and immunofluorescence on formalin fixed, paraffin embedded tisues sections.Image caption:PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick and healthy sheep as a control for PET blot analysis procedure. Scale bar = 100 µm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1 cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100 µm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla as well as in the papillae of kidneys derived from naturally scrapie infected sheep. Scale bar = 30 µm.From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep. PLoS ONE 2(9): e859.
  • IHC-P - Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11  ABD11789
    Published customer image:Mouse anti Sheep CD230 antibody, clone 2G11 used for the detection of prion protein in ovine brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption: Immunohistochemistry of brain in donor and recipient sheep. Trigeminal nucleus from donor (A) and recipient (B) animals; intraneuronal PrPSc is evident only in the recipient animal (B). White matter from donor (C) and recipient (D) animals; globular white matter labelling is evident only in the donor animal (C). Scale bar in (A) and (B) indicates 100 µm; in (C) and (D) indicates 50 µm. Antibody 2G11From: Simmons MM, Moore SJ, Lockey R, Chaplin MJ, Konold T, et al. Phenotype Shift from Atypical Scrapie to CH1641 following Experimental Transmission in Sheep. PLoS ONE 10(2): e0117063.
  • IHC-P - Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11  ABD11789
    Published customer image:Mouse anti sheep CD230 antibody, clone 2G11 used for the detection of prion protein in ovine brain by paraffin embedded tissue blot analysis.Image caption:PET blot analysis identifying PrPSc in the tubular structures of the cortex and medulla in naturally scrapie-sick Sarda sheep. (A) Brains of scrapie-sick and healthy sheep as a control for PET blot analysis procedure. Scale bar = 100 µm. (B) Low magnification of transverse paraffin sections from kidneys of a naturally scrapie-sick and a healthy sheep demonstrating PrPSc in the medullary and cortical regions of the kidney. Dotted lines indicate the locus of the kidney capsule or the edge of the tissue section. The small transparent box indicates the region shown at higher magnification and resolution in figure C. Scale bar = 1 cm. (C) PrPSc deposits and tubular structure fit together in the cortical part of the kidney in scrapie-sick sheep. Scale bar = 100 µm. (D) PrPSc is also found to a high degree in the collecting ducts of the medulla as well as in the papillae of kidneys derived from naturally scrapie infected sheep. Scale bar = 30 µm.From: Ligios C, Cancedda GM, Margalith I, Santucciu C, Madau L, et al. Intraepithelial and Interstitial Deposition of Pathological Prion Protein in Kidneys of Scrapie-Affected Sheep. PLoS ONE 2(9): e859.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, E
Primary Accession P23907
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 2G11
Calculated MW 27915 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Synthetic peptide 146-R154R171-182 of ovine PrP.
Shelf Life 18 months from date of despatch.
Gene ID 493887
Other Names Major prion protein, PrP, CD230, PRNP, PRP, SIP
Target/Specificity Mouse anti-Sheep CD230 (Prpsc) antibody, clone 2G11 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. ABD11789 is specific for PrPSc. This clone does not appear to immunostain the normall cellular PrP expressed in heathly sheep tissue sections resulting in specific labelling of ovine PrPSc. This antibody was raised against a synthetic peptide (146-R154R171-182 ) of the ovine PrP peptide and specificially recognises the R151-R159sequence.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Sheep CD230 (Prpsc) Antibody, clone 2G11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

References

1. Andréoletti, O. et al. (2000) Early accumulation of PrP(Sc) in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie.
J Gen Virol. 81 (Pt 12): 3115-26. 2. Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.
J Histochem Cytochem. 50 (10): 1357-70. 3. Ortiz-Pelaez, A. et al. (2015) Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in Cypriot goats.
Epidemiol Infect. 143 (6): 1304-10. 4. Simmons, M.M. et al. (2015) Phenotype shift from atypical scrapie to CH1641 following experimental transmission in sheep.
PLoS One. 10 (2): e0117063.

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD11789
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 400+ Stem Cells Antibodies. PromoCode: <span class=text-red>FLASH19</span>
Terms & Conditions