|Application ||IHC-P, E|
|Calculated MW||27915 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide 146-R154R171-182 of ovine PrP.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Major prion protein, PrP, CD230, PRNP, PRP, SIP|
|Target/Specificity||Mouse anti-Sheep CD230 (Prpsc) antibody, clone 2G11 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. ABD11789 is specific for PrPSc. This clone does not appear to immunostain the normall cellular PrP expressed in heathly sheep tissue sections resulting in specific labelling of ovine PrPSc. This antibody was raised against a synthetic peptide (146-R154R171-182 ) of the ovine PrP peptide and specificially recognises the R151-R159sequence.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Sheep CD230 (Prpsc) Antibody, clone 2G11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Andréoletti, O. et al. (2000) Early accumulation of PrP(Sc) in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie.J Gen Virol. 81 (Pt 12): 3115-26. 2. Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.J Histochem Cytochem. 50 (10): 1357-70. 3. Ortiz-Pelaez, A. et al. (2015) Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in Cypriot goats.Epidemiol Infect. 143 (6): 1304-10. 4. Simmons, M.M. et al. (2015) Phenotype shift from atypical scrapie to CH1641 following experimental transmission in sheep.PLoS One. 10 (2): e0117063.
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