|Application ||WB, IF, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Bacterial TrpE-LMP2A fusion protein|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Epstein-Barr Virus LMP2A antibody, clone 14B7 recognizes latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV). EBV is a human herpesvirus, which is associated with conditions such as Hodgkin’s disease and Burkitt’s Lymphoma and is the causative agent in mononucleosis in adolescents.EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.Clone 14B7 detects wild-type LMP2A protein.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Epstein-Barr Virus LMP2A Antibody, clone 14B7 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Fruehling, S. et al. (1996) Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction.J Virol. 70:6216 – 6226. 2. Fruehling, S. et al. (1998) Tyrosine 112 of latent membrane protein 2A is essential for protein tyrosine kinase loading and regulation of Epstein-Barr virus latency.J Virol. 72:7796 – 7806. 3. Ikeda, A. et al. (2003) Itchy, a Nedd4 ubiquitin ligase, downregulates latent membrane protein 2A activity in B-cell signaling.J Virol. 77: 5529-34. 4. Longan, L. and Longnecker, R. (2000) Epstein-Barr virus latent membrane protein 2A has no growth-altering effects when expressed in differentiating epithelia.J Gen Virol. 81: 2245-52. 5. Lynch, D.T. et al. (2002) Epstein-Barr virus latent membrane protein 2B (LMP2B) co-localizes with LMP2A in perinuclear regions in transiently transfected cells.J Gen Virol. 83: 1025-35. 6. Bultema, R. et al. (2009) Epstein-Barr virus LMP2A accelerates MYC-induced lymphomagenesis.Oncogene. 28: 1471-6. 7. Katzman, R.B. and Longnecker, R. (2004) LMP2A does not require palmitoylation to localize to buoyant complexes or for function.J Virol. 78: 10878-87. 8. Anderson, L.J. and Longnecker, R. (2008) An auto-regulatory loop for EBV LMP2A involves activation of Notch.Virology. 371: 257-66. 9. Merchant, M. et al. (2000) The LMP2A ITAM is essential for providing B cells with development and survival signals in vivo.J Virol. 74: 9115-24. 10. Bieging, K.T. et al. (2009) Epstein-Barr virus LMP2A bypasses p53 inactivation in a MYC model of lymphomagenesis.Proc Natl Acad Sci USA 106: 17945-50. 11. Caldwell, R.G. et al (2000) Epstein-Barr virus LMP2A-induced B-cell survival in two unique classes of EmuLMP2A transgenic mice.J Virol. 74: 1101-13. 12. Moody, C.A. et al. (2005) Modulation of the cell growth regulator mTOR by Epstein-Barr virus-encoded LMP2A.J Virol. 79: 5499-506. 13. Rovedo, M. and Longnecker, R. (2007) Epstein-barr virus latent membrane protein 2B (LMP2B) modulates LMP2A activity.J Virol. 81: 84-94. 14. Sim, A.C. et al. (2013) Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.Sci Rep. 3: 3232.
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