|Application ||FC, IP|
|Clone Names||Du CD4-2|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||293T cells expressing Pekin duck CD4.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Duck CD4 antibody, clone Du CD4-2 recognizes Pekin duck CD4, shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells (Kothlowet al.2005).Clone Du CD4-2 can be used to identify duck T helper cells. This antibody has been shown to not react with Mallard.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Duck CD4 Antibody, clone Du CD4-2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.Dev. and Comp. Immunol. 29: 733-748. 2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.Vet Res. 43: 56. 3. Shanmugasundaram, R. and Selvaraj, R.K. (2012) Regulatory T cell properties of thymic CD4(+)CD25(+) cells in ducks.Vet Immunol Immunopathol. 149: 20-7. 4. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.Virol J. 8: 214. 5. Huang, J. net al. (2014) An attenuated duck plague virus (DPV) vaccine induces both systemic and mucosal immune responses to protect ducks against virulent DPV infection.Clin Vaccine Immunol. 21: 457-62. 6. Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.Immunobiology. pii: S0171-2985(14)00289-7. 7. Zhou, H. et al. (2016) LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In VitroandIn VivoStudiesFrontiers in Microbiology. Feb 17: [Epub ahead of print]1. Higgins, D.A. and Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.J. Immunol. Methods. 21: 135-145.
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