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>   home   >   Products   >   Primary Antibodies   >   Anti-Duck CD8 alpha Antibody, clone Du CD8-1    

Anti-Duck CD8 alpha Antibody, clone Du CD8-1

Mouse Anti-Duck Monoclonal Antibody

  • FC - Anti-Duck CD8 alpha Antibody, clone Du CD8-1  ABD11794
    Published customer image:T lymphocytes in PBL following DEV UL24 DNA vaccination. 1, 2, 3, 4, 6, 8weeks after vaccination, the isolated PBL were stained with monoclonal antibodies against duck CD4 (A) and CD8 (B). The results presented are the mean of all specimens of each group. Statistical significance was determined using a student’st-test.From: Yu X, Jia R, Huang J, Shu B, Zhu D, Liu Q, Gao X, Lin M, Yin Z, Wang M, Chen S, Wang Y, Chen X, Cheng A. Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge. Vet Res. 2012 Jul 6;43:56.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names Du CD8-1
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen 293T cells expressing Pekin Duck CD8 alpha.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).Double-staining with clone Du CD8-1 and MCA2480 Mouse anti-Duck IgY light chain clone 14A3 revealed the presence of a CD8high/14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlowet al.1985). Mouse anti-Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Duck CD8 alpha Antibody, clone Du CD8-1 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
Dev Comp Immunol. 29 (8): 733-48. 2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
Vet Res. 43: 56. 3. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
Virol J. 8: 214. 5. Chen, S. et al. (2015) Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.
Poult Sci. 94 (1): 17-24.1. Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
J Immunol Methods. 106 (1): 135-45.

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Cat# ABD11794
(40 western blots)
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