|Application ||WB, IHC-P, E|
|Calculated MW||11025 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant human MCP-1/MCAF ( PHP061)|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2, CCL2, MCP1, SCYA2|
|Target/Specificity||Mouse anti-Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils (Weberet al.1996).MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells (Cushinget al.1990). MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype (Karpus and Kennedy 1997). Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection (Flores-Villanuevaet al.2005).Mouse anti-Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes both native and recombinant MCP-1 in conjunction with Rabbit anti-Human MCP-1 polyclonal antibody in a sensitive sandwich ELISA assay.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human MCP-1 Antibody, clone 2.2-4A4-1A11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Johansson, U. et al. (2008) Formation of composite endothelial cell-mesenchymal stem cell islets: a novel approach to promote islet revascularization. Diabetes. 57: 2393-401. 2. Nio-Kobayashi J et al. (2015) Regulated C-C motif ligand 2 (CCL2) in luteal cells contributes to macrophage infiltration into the human corpus luteum during luteolysis.Mol Hum Reprod. pii: gav028.
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