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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human PI-9 Antibody, clone 7D8    

Anti-Human PI-9 Antibody, clone 7D8

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50453
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 7D8
Calculated MW 42404 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant PI-9 produced in P. pastoris.
Shelf Life 18 months from date of despatch.
Gene ID 5272
Other Names Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Peptidase inhibitor 9, PI-9, SERPINB9, PI9
Target/Specificity Mouse anti-Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells.Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Clone 7D8 has been reported to work in western blotting applications. Serotec recommend the use of ABD11805 for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirstet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human PI-9 Antibody, clone 7D8 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Hirst, C.E. et al. (2001) Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction.
Mol Hum Reprod. 7: 1133-42. 2. Hirst, C.E. et al. (2003) The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency.
J. Immunol. 170: 805-815. 3. Heutinck, K.M. et al. (2012) SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I
Nephrol Dial Transplant. 27: 2746-54. 4. Buzza, M.S. et al. (2001) The granzyme B inhibitor, PI-9, is present in endothelial and mesothelial cells, suggesting that it protects bystander cells during immune responses.
Cell Immunol. 210: 21-9. 5. Pohjanen VM et al. (2013) Decreased expression of protease inhibitor 9, a granzyme B inhibitor, in celiac disease: a potential mechanism in enterocyte destruction and villous atrophy.
Int J Immunopathol Pharmacol. 26 (4): 897-905.

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Cat# ABD11805
(40 western blots)
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