|Application ||WB, IHC-P, IHC-F, FC|
|Calculated MW||42404 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant PI-9 produced in P. pastoris.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Peptidase inhibitor 9, PI-9, SERPINB9, PI9|
|Target/Specificity||Mouse anti-Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells.Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Clone 7D8 has been reported to work in western blotting applications. Serotec recommend the use of ABD11805 for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirstet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human PI-9 Antibody, clone 7D8 is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Hirst, C.E. et al. (2001) Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction.Mol Hum Reprod. 7: 1133-42. 2. Hirst, C.E. et al. (2003) The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency. J. Immunol. 170: 805-815. 3. Heutinck, K.M. et al. (2012) SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I Nephrol Dial Transplant. 27: 2746-54. 4. Buzza, M.S. et al. (2001) The granzyme B inhibitor, PI-9, is present in endothelial and mesothelial cells, suggesting that it protects bystander cells during immune responses.Cell Immunol. 210: 21-9. 5. Pohjanen VM et al. (2013) Decreased expression of protease inhibitor 9, a granzyme B inhibitor, in celiac disease: a potential mechanism in enterocyte destruction and villous atrophy.Int J Immunopathol Pharmacol. 26 (4): 897-905.
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