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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   Anti-Human Podoplanin Antibody, clone D2-40    

Anti-Human Podoplanin Antibody, clone D2-40

Mouse Anti-Human Monoclonal Antibody

     
  • WB - Anti-Human Podoplanin Antibody, clone D2-40  ABD11807
    Published customer image:Mouse anti Human podoplanin antibody, clone D2-40 used for the detection of podoplanin in TCP1 cells before and following podoplanin silencing with siRNA by western blotting and immunofluorescence.Image caption:Down-regulation of podoplanin expression in TPC1 cells following transfection with PDPN-specific siRNA. A. RT-qPCR analysis of PDPN mRNA levels in TPC1 thyroid cancer cells 48 h after transfection with 30 nM siRNA specific for PDPN (siPDPN) or a negative control siRNA (siNEG). The results were normalized to the 18S rRNA level and bars represent the average fold change in PDPN transcript abundance in cells transfected with siPDPN compared with cells transfected with siNEG. The results are representative of four independent experiments. Data are presented as the mean ±SEM, **P
  • WB - Anti-Human Podoplanin Antibody, clone D2-40  ABD11807
    Published customer image:Mouse anti Human podoplanin antibody, clone D2-40 used for the detection of podoplanin in human thyroid cancer derived cell lines by western blotting and immunofluorescence.Image caption:Podoplanin transcript and protein expression levels in differentiated thyroid cancer derived cell lines. A. PDPN mRNA expression in human thyroid cancer cell lines. RT-qPCR was used to evaluate levels of the transcript encoding podoplanin in total RNA prepared from NTHY control cells, PTC-derived TPC1 and BcPAP cell lines, and FTC-derived FTC133 and CGTH-W-1 cell lines. Data from four independent experiments performed in triplicate are expressed as the mean ±SEM, **P
  • IHC-P - Anti-Human Podoplanin Antibody, clone D2-40  ABD11807
    Published customer image:Mouse anti Human Podoplanin antibody, clone D2-40 used for the detection of podoplanin in human synovial tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Podoplanin is expressed in FLS in areas of synovial transformation. Immunohistochemistry of (A and B) frozen and (C-J) paraffin-embedded human synovial tissue from patients with rheumatoid arthritis using antibodies against (A and C) podoplanin , (B) CD90 and (D) cadherin-11 . Double immunofluorescence staining analysed by confocal microscopy showing, in green, (E) podoplanin 18H5 and (F-J) podoplanin D2-40, and in red, (E) cadherin-11 , (F) CD68 , (G and overview in H) E-cadherin (H-108), (I) a-smooth muscle actin (a-sma) and (J) matrix metalloproteinase 9 (MMP-9). (E) Note colocalization of podoplanin and cadherin-11 . L, lumen.From: Ekwall AK, Eisler T, Anderberg C, Jin C, Karlsson N, Brisslert M, Bokarewa MI. The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis. Arthritis Res Ther. 2011 Mar 7;13(2):R40.
  • WB - Anti-Human Podoplanin Antibody, clone D2-40  ABD11807
    Published customer image:Mouse anti Human Podoplanin antibody, clone D2-40 used for the detection of podoplanin in human synovial tissue by western blotting.Image caption:Anti-podoplanin antibody D2-40 recognizes 45kD band in Western blot of synovial protein extracts. Western blot of extracted membrane proteins from human synovial tissue from a patient with rheumatoid arthritis (lane 1), cell pellet of human MRC-5 lung fibroblast cell line (lane 2) and recombinant immature podoplanin core protein (lane 3) separated on a 20% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel probed with the antipodoplanin antibody . From: Ekwall AK, Eisler T, Anderberg C, Jin C, Karlsson N, Brisslert M, Bokarewa MI. The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis. Arthritis Res Ther. 2011 Mar 7;13(2):R40.
  • IHC-P - Anti-Human Podoplanin Antibody, clone D2-40  ABD11807
    Published customer image:Mouse anti Human podoplanin antibody, clone D2-40 used for the detection of podoplanin in human thyroid tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Podoplanin expression in human thyroid tissues analyzed by immunohistochemistry.Immunohistochemistry detection of podoplanin was carried on paraffin-embedded tissue sections. Representative immunostaining results obtained with anti-PDPN monoclonal antibody D2-40. (a) exclusive and strong staining of lymphatic vessels in normal thyroid tissue; (b) follicular adenoma tissue negative for podoplanin; (c) follicular thyroid carcinoma tissue negative for podoplanin; (d) papillary thyroid carcinoma case negative for podoplanin; (e-l) intense cytoplasmic staining of PDPN in PTC cells. In e, j and i intense staining of PDPN in tumor cells, with the peritumoral margin negative for podoplanin and strong staining of lymphatic vessels as an internal positive control. Original magnification: a-100x, a-inset -100x; b-200x, b-inset -100x; c-200x; d-200x, e-100x, f-100x, g-200x; h-200x, i-100x, j-200x, k-200x, l-400x. Of 112 PTC cases, 45 (40%) displayed ectopic podoplanin expression in the cancer cells.From: Rudzińska M, Gawel D, Sikorska J, Karpińska KM, Kiedrowski M, Stepień T, et al. The Role of Podoplanin in the Biology of Differentiated Thyroid Cancers.PLoS ONE 9(5): e96541.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q86YL7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names D2-40
Calculated MW 16698 Da
Additional Information
Immunogen Resected tissue from dysgeminoma of the ovary
Shelf Life 18 months from date of despatch.
Gene ID 10630
Other Names Podoplanin, Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A, PDPN {ECO:0000312|EMBL:AAH14668.2}
Target/Specificity Mouse anti-human podoplanin antibody, clone D2-40 was raised against M2A antigen (Markset al, 1999) and detects podoplanin (Sonneet al, 2006). Podoplanin (PDPN) is an O-glycosylated transmembrane glycoprotein that is selectively expressed by, and is a marker of, lymphatic endothelial cells. In normal tissue the 38 kDa protein is also present in human lung, placenta, heart, skeletal muscle and kidney podocytes. It is not found in the blood vasculature (Breiteneder-Geleffet al, 1999,Wicki and Christofori, 2007). The function of podoplanin is yet to be fully elucidated; however, it may be involved in cell migration and/or actin cytoskeleton organization. It is required for normal lung cell proliferation and alveolus formation at birth, and can induce platelet aggregation (Ramirezet al, 2003,Wicki and Christofori, 2007).Mouse anti-human podoplanin antibody, clone D2-40 has been shown to be a sensitive and specific antibody for the detection of lymphatic endothelium in different malignancies, and is of value in the routine evaluation of lymphatic invasion in esophageal cancer (Kozłowskiet al, 2011). Clone D2-40 was reported to be an excellent immunohistochemical marker of cutaneous Kaposi’s sarcomas, (Kahnet al, 2002), and may be useful in the differential diagnosis of epithelioid malignant mesothelioma versus adenocarcinoma (Chuet al, 2005).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Podoplanin Antibody, clone D2-40 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Marks, A. et al. (1999) Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours.
Br J Cancer. 80 (3-4): 569-78. 2. Wu, H.M. et al. (2013) Expression of podoplanin in salivary gland adenoid cystic carcinoma and its association with distant metastasis and clinical outcomes.
Mol Med Rep. 6: 271-4. 3. Takagi, S. et al. (2013) Platelets promote tumor growth and metastasis via direct interaction between Aggrus/podoplanin and CLEC-2.
PLoS One. 8: e73609. 4. Sonne, S.B. et al. (2006) Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours.
Virchows Arch. 449 (2): 200-6. 5. Heindl, L.M. et al (2010) Intraocular tumor-associated lymphangiogenesis a novel prognostic factor for ciliary body melanomas with extraocular extension.
Ophthalmology 117(2): 334-42. 6. Ekwall, A.K. et al. (2011) The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the hyperplastic synovial lining layer in rheumatoid arthritis.
Arthritis Res Ther. 13:R40. 7. Kozłowski, M. et al. (2011) Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer.
Folia Histochem Cytobiol. 49: 90-7. 8. Rudzińska, M. et al. (2014) The role of podoplanin in the biology of differentiated thyroid cancers.
PLoS One. 9: e96541. 9. Bolzoni Villaret, A. et al. (2010) Immunostaining patterns of CD31 and podoplanin in previously untreated advanced oral/oropharyngeal cancer: prognostic implications.
Head Neck. 32: 786-92. 10. Heindl, L.M. et al. (2014) Lack of ciliary body lymphatics in iridociliary melanocytoma.
Graefes Arch Clin Exp Ophthalmol. 252: 169-71. 11. Heindl, L.M. et al. (2009) Intraocular lymphangiogenesis in malignant melanomas of the ciliary body with extraocular extension.
Invest Ophthalmol Vis Sci. 50: 1988-95. 12. Heindl, L.M. et al. (2010) Intraocular tumor-associated lymphangiogenesis a novel prognostic factor for ciliary body melanomas with extraocular extension?
Ophthalmology. 117: 334-42. 13. Wessel, J.M. et al. (2012) Invasion of lymphatic vessels into the eye after open globe injuries.
Invest Ophthalmol Vis Sci. 53: 3717-25. 14. Heindl, L.M. et al. (2011) Tumor-associated lymphangiogenesis in the development of conjunctival melanoma.
Invest Ophthalmol Vis Sci. 52: 7074-83. 15. Bolzoni Villaret, A. et al. (2013) Angiogenesis and lymphangiogenesis in early-stage laryngeal carcinoma: Prognostic implications.
Head Neck. 35: 1132-7. 16. Heindl, L.M. et al. (2011) Prognostic significance of tumor-associated lymphangiogenesis in malignant melanomas of the conjunctiva.
Ophthalmology. 118: 2351-60. 17. Miyata, K. et al. (2014) Suppression of Aggrus/podoplanin-induced platelet aggregation and pulmonary metastasis by a single-chain antibody variable region fragment.
Cancer Med. 3 (6): 1595-604.

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