Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD28 Antibody, clone D665    

Anti-Mouse CD28 Antibody, clone D665

Mouse Anti-Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31041
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names D665
Calculated MW 25243 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Alternative immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Shelf Life 18 months from date of despatch.
Gene ID 12487
Other Names T-cell-specific surface glycoprotein CD28, CD28, Cd28
Target/Specificity Mouse anti-Mouse CD28 antibody, clone D665 recognizes murine CD28, also known as T-cell-specific surface glycoprotein CD28, a 218 amino acid ~90 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like v-type domain. CD28 is a member of the immunoglobulin superfamily and is structurally related to CD152 (CTLA-4). CD28 is a major costimulatory molecule, expressed by most T cells including thymocyte subsets and peripheral T-cells but is absent on a subset of CD8+ve cells. Engagement of CD28 by its natural ligand, CD80 or CD86, provides a costimulatory signal which promotes T-cell effector function and T-cell-dependent antibody production. Mouse anti-mouse CD28 antibody clone D665 is reported to act as a CD28 superagonist antibody, which induces antigen-independent T cell proliferation (Dennehyet al.2006). Mouse anti-mouse CD28 antibody, clone D665 binds to an epitope in the C"D loop of murine CD28, similar to the recognized epitope for other superagonist antibodies directed against human and rat CD28 targets (Lühderet al.2003).Activation of Treg cells through engagement of CD28 by mouse anti-mouse CD28 antibody, clone D665 leads to improved survival and healing in mice folowing myocardial infarction by modulating monocyte/ macrophage differentiation (Weiratheret al.2014). In mice it appears that Treg cells offer sufficient regulatory control following superagonist engagement of anti-CD28 antibody to dampen the proinflammatory cytokine release, seen in the absence of Treg cells (Gogishviliet al.2009) and in the cytokine release syndrome observed in participants of the phase 1 CD28 superagonist antibody TGN1412 clinical trial (Suntharalingamet al.2006).
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Mouse CD28 Antibody, clone D665 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


1. Dennehy, K.M. et al. (2006) Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.
J Immunol. 176 (10): 5725-9. 2. Vaeth, M. et al, (2012) Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells.
Proc Natl Acad Sci U S A. 109: 16258-63. 3. Gogishvili, T. et al. (2009) Rapid regulatory T-cell response prevents cytokine storm in CD28 superagonist treated mice.
PLoS One. 4: e4643. 4. Zhang, T. et al. (2011) Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner.
Am J Transplant. 11: 1599-609. 5. Weirather, J. et al. (2014) Foxp3+ CD4+ T cells improve healing after myocardial infarction by modulating monocyte/macrophage differentiation.
Circ Res. 115 (1): 55-67.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# ABD11818
(40 western blots)
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions