|Calculated MW||25243 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Alternative immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell-specific surface glycoprotein CD28, CD28, Cd28|
|Target/Specificity||Mouse anti-Mouse CD28 antibody, clone D665 recognizes murine CD28, also known as T-cell-specific surface glycoprotein CD28, a 218 amino acid ~90 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like v-type domain. CD28 is a member of the immunoglobulin superfamily and is structurally related to CD152 (CTLA-4). CD28 is a major costimulatory molecule, expressed by most T cells including thymocyte subsets and peripheral T-cells but is absent on a subset of CD8+ve cells. Engagement of CD28 by its natural ligand, CD80 or CD86, provides a costimulatory signal which promotes T-cell effector function and T-cell-dependent antibody production. Mouse anti-mouse CD28 antibody clone D665 is reported to act as a CD28 superagonist antibody, which induces antigen-independent T cell proliferation (Dennehyet al.2006). Mouse anti-mouse CD28 antibody, clone D665 binds to an epitope in the C"D loop of murine CD28, similar to the recognized epitope for other superagonist antibodies directed against human and rat CD28 targets (Lühderet al.2003).Activation of Treg cells through engagement of CD28 by mouse anti-mouse CD28 antibody, clone D665 leads to improved survival and healing in mice folowing myocardial infarction by modulating monocyte/ macrophage differentiation (Weiratheret al.2014). In mice it appears that Treg cells offer sufficient regulatory control following superagonist engagement of anti-CD28 antibody to dampen the proinflammatory cytokine release, seen in the absence of Treg cells (Gogishviliet al.2009) and in the cytokine release syndrome observed in participants of the phase 1 CD28 superagonist antibody TGN1412 clinical trial (Suntharalingamet al.2006).|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Mouse CD28 Antibody, clone D665 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Dennehy, K.M. et al. (2006) Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.J Immunol. 176 (10): 5725-9. 2. Vaeth, M. et al, (2012) Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells.Proc Natl Acad Sci U S A. 109: 16258-63. 3. Gogishvili, T. et al. (2009) Rapid regulatory T-cell response prevents cytokine storm in CD28 superagonist treated mice.PLoS One. 4: e4643. 4. Zhang, T. et al. (2011) Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner.Am J Transplant. 11: 1599-609. 5. Weirather, J. et al. (2014) Foxp3+ CD4+ T cells improve healing after myocardial infarction by modulating monocyte/macrophage differentiation.Circ Res. 115 (1): 55-67.
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