|Application ||WB, IHC-F, FC, IP|
|Calculated MW||109135 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine bone marrow cells (BMC).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mast/stem cell growth factor receptor Kit, SCFR, 188.8.131.52, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, KIT|
|Target/Specificity||Mouse anti-Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perezet al.2007). Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD). Mouse anti-pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig CD117 Antibody, clone 2B8/BM is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Pérez C et al. (2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.J Immunol Methods. 321 (1-2): 70-9. 2. Hatzistergos, K.E. et al. (2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.Circ Res. 107: 913-22. 3. Escalona, Z. et al. (2014) Molecular characterization and expression of porcine Siglec-5.Dev Comp Immunol. 44 (1): 206-16.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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