|Application ||WB, E, Func|
|Clone Names||046-10.2.2 (10-04)|
|Calculated MW||188305 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Purified human C5|
|Shelf Life||18 months from date of despatch.|
|Other Names||Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5 beta chain, Complement C5 alpha chain, C5a anaphylatoxin, Complement C5 alpha' chain, C5, CPAMD4|
|Target/Specificity||ABD11833 recognises secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. In haemolysis inhibition studies it was found that ABD11833 will block C5 haemolytic activity. Removal of sodium azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human C5 Antibody, clone 046-10.2.2 (10-04) is for research use only and not for use in diagnostic or therapeutic procedures.|
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