|Application ||WB, IHC-F, FC, E|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Human SC5b-9 antibody, clone 3R2/0 recognizes the SC5b-9 complex of around 330 kDa. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.Removal of Sodium Azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose. Mouse anti-Human SC5b-9 antibody, clone 3R2/0 has been reprted to work in western blotting applications.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human SC5b-9 Antibody, clone 3R2/0 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Biesecker, G. (1990) The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor. J. Immunol. 145:209. 2. Greenstein, J. et al. (1995) The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation. Clin. Exp. Immunol.100:40-6. 3. Rus H, et al. (2005) C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection. Ann Med. 37:97-104.
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