|Application ||WB, IHC-F, FC, E|
|Calculated MW||52495 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Glomerular basement membrane from a post-mortem kidney.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, CLI, Complement-associated protein SP-40, 40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, TRPM-2, Clusterin beta chain, ApoJalpha, Complement cytolysis inhibitor a chain, Clusterin alpha chain, ApoJbeta, Complement cytolysis inhibitor b chain, CLU, APOJ, CLI, KUB1|
|Target/Specificity||ABD11835 recognises clusterin, an ubiquitously expressed protein of two 40-kD chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localised to the nucleus and a pro-survival secreted form.Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas. Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer’s disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Clusterin Antibody, clone 3R3/2 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kumita, J. et al. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species.J. Mol. Biol. 369:157-167. 2. Shannan, B. et al. (2006) Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control. J. Mol. Histol. 37:183-188.
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