|Application ||IHC-P, IHC-F, E|
|Calculated MW||50246 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Other Names||Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, TERF1, PIN2, TRBF1, TRF, TRF1|
|Target/Specificity||Mouse anti-human TRF1 antibody, clone BED5 recognizes TTAGGG repeat binding factor 1 (TRF1) a ubiquitously expressed 60kDa protein. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage. TRF1 is thought to play a role in leukaemia being expressed at significantly higher levels in patients with acute lymphoblastic leukaemia than those with myeloid leukaemia (Ohyashikiet al.2001).Mouse anti-human TRF1 antibody, clone BED5 has been reported to work in Western blotting applications.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human TRF1 Antibody, clone BED5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Galati, A. et al. (2006) The human telomeric protein TRF1 specifically recognizes nucleosomal binding sites and alters nucleosome structure.J. Mol. Biol. 360: 377-85. 2. de Lange, T. et al (2005) Shelterin: the protein complex that shapes and safeguards human telomeres.Genes Dev. 19: 2100-10.
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