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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Human BUB1 Antibody, clone 14H5    

Anti-Human BUB1 Antibody, clone 14H5

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43683
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 14H5
Calculated MW 122375 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant protein corresponding to amino acids 281-419 of human Bub1 protein.
Shelf Life 18 months from date of despatch.
Gene ID 699
Other Names Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1,, BUB1A, BUB1, BUB1L
Target/Specificity ABD11865 detects Bub1, a protein kinase involved in spindle checkpoint function during mitosis. Bub1 co-operates with Aurora B kinase to inhibit Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align on the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelity mitotic chromosome segregation. Bub1 is also required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshin protein, and metaphase congression. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human BUB1 Antibody, clone 14H5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Morrow, C.J. et al. (2005) Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20.
J. Cell Sci. 118: 3639-3652. 2. Meraldi, P. and Sorger, P.K. (2005) A dual role for Bub1 in the spindle checkpoint and chromosome congression.
EMBO J. 24: 1621-1633. 3. Vanoosthuyse, V. and Hardwick, K.G. (2005) Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis.
Trends Cell Biol. 15: 231-233.

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Cat# ABD11865
(40 western blots)
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