|Application ||E, ELISPOT|
|Calculated MW||19393 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified recombinant bovine IFN gamma expressed in E.coli.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon gamma, BoIFNG, IFN-gamma, IFNG|
|Target/Specificity||Mouse anti-Bovine Interferon Gamma (IFNγ), clone 5D10, recognises the 19.3kDa protein belonging to the type II interferon family .Bovine IFNγ is produced by activated lymphocytes, has anti-viral and immunoregulatory properties and is an activator of macrophages. Clone 5D10 is one of a range of bovine IFNγ reagents available from AbD serotec which include the monoclonal antibody clones CC330, CC302 and 7B6 along with recombinant bovine IFNγ and bovine IFNγ ELISA kits. Clone 5D10 is suitable for ELISA and ELISpot, for intracellular identification of bovine IFNγ and may be used in conjuction with clone CC302 (clone CC302 is available in a range of direct conjugates) as a detection reagent.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine Interferon gamma Antibody, clone 5D10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Walravens, K. et al. (2002) Analysis of the antigen-specific IFN-gamma producing T-cell subsets in cattle experimentally infected with Mycobacterium bovis.Vet. Immunol. Immunopathol. 84: 29-41. 2. Lemaire, M. et al. (2000) Effects of bovine Herpesvirus type 1 infection in calves with maternal antibodies on immune response and virus latency. J. Clin. Microbiol. 38: 1885-1894. 3. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1+ gammadelta T cells. Infect. Infect Immun. 70: 1488-1500. 4. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle. Vet. Immunol. Immunopathol. 66: 309-320.
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