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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human Adiponectin Antibody, clone 5H7    

Anti-Human Adiponectin Antibody, clone 5H7

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human Adiponectin Antibody, clone 5H7  ABD11867
    Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown
  • E - Anti-Human Adiponectin Antibody, clone 5H7  ABD11867
    Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 is shown
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15848
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 5H7
Calculated MW 26414 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant human adiponectin (amino acids 15-244) purified from Escherichia coli .
Shelf Life 18 months from date of despatch.
Gene ID 9370
Other Names Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, ACRP30, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28
Target/Specificity Mouse anti-Human adiponectin, clone 5H7 recognizes the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848).Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes (Heilbronnet al.2011). Mutations in the adiponectin gene can also lead to adiponectin deficiency charactrized by obesity, diabetes, high blood pressure and circulating cholerterol (Takahashiet al.2000).Mouse anti-Human adiponectin, clone 5H7 had been used succesfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti-adiponectin clones Adn27orAdn36 as capture reagents.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Adiponectin Antibody, clone 5H7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Lihn, A.S. et al. (2005) Adiponectin: action, regulation and association to insulin sensitivity.
Obes. Rev. 6: 13-21.2. Trayhurn, P. and Wood, I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity.
Biochem. Soc. Trans. 33: 1078-1081.

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Cat# ABD11867
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