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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Hexokinase 1 Antibody, clone 4D7    

Anti-Human Hexokinase 1 Antibody, clone 4D7

Mouse Anti-Human Monoclonal Antibody

  • IF - Anti-Human Hexokinase 1 Antibody, clone 4D7  ABD11870
    Immunofluorescent staininf of HeLa Cells with Mouse anti Human hexokinase 1 , green. Counterstained nuclei with Hoechst 33342, blue
  • WB - Anti-Human Hexokinase 1 Antibody, clone 4D7  ABD11870
    Western blotting analysis of hexokinase specificity using Mouse anti Human hexokinase 1, clone 4D7 A: hexokinase specificity against recombinant hexokinase proteinsB: hexokinase expression in human cell lines and mouse brain
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19367
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 4D7
Calculated MW 102486 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant human hexokinase 1 (amino acids 1-917) purified from Escherichia coli.
Shelf Life 18 months from date of despatch.
Gene ID 3098
Other Names Hexokinase-1,, Brain form hexokinase, Hexokinase type I, HK I, HK1
Target/Specificity Mouse anti-Human hexokinase 1, clone 4D7 recogniszs human hexokinase 1, a 102kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia (Newmanet al.1980).Mouse anti-hexokinase 1, clone 4D7 recognizes an epitope common to all isoforms of human hexokinase.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Hexokinase 1 Antibody, clone 4D7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Wilson, J.E. (2003) Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function.
J. Exp. Biol. 206: 2049-2057.

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Cat# ABD11870
(40 western blots)
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