|Application ||WB, E, IP|
|Calculated MW||47219 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Recombinant human IRF3 (amino acids 108-166) purified from E. coli.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon regulatory factor 3, IRF-3, IRF3|
|Target/Specificity||Mouse anti-Human IRF3 antibody, clone 3F10 recognizes human interferon regulatory factor 3 (IRF3), a 55kDa constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity (Nociariet al.2007).IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta and subsequent downstream interferon-induced genes (Weaveret al.1998).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human IRF3 Antibody, clone 3F10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Takahasi, K. et al. (2003) X-ray crystal structure of IRF-3 and its functional implications. Nat. Struc. Biol. 10: 922-927.
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