|Application ||WB, E, IP|
|Calculated MW||47219 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Recombinant human IRF3 (amino acids 108-166) purified from E. coli.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interferon regulatory factor 3, IRF-3, IRF3|
|Target/Specificity||Mouse anti-Human IRF3 antibody, clone 3F10 recognizes human interferon regulatory factor 3 (IRF3), a 55kDa constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity (Nociariet al.2007).IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta and subsequent downstream interferon-induced genes (Weaveret al.1998).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human IRF3 Antibody, clone 3F10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Takahasi, K. et al. (2003) X-ray crystal structure of IRF-3 and its functional implications. Nat. Struc. Biol. 10: 922-927.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.