|Application ||WB, IHC-P, IP|
|Calculated MW||51386 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||HeLa cell cytoskeleton preparation.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL|
|Target/Specificity||ABD11873 specifically recognises a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kDa type II basic cytokeratin, and 46kDa type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. ABD11873 is reported as suitable for use in immunocytochemistry, see Bartek, J. et al. for details.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Cytokeratin 7/17 Antibody, clone C-46 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bartek, J. et al. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164: 215-224. 2. Vojtesek, B. et al. (1990) A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 37: 333-342.
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