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Mouse Anti-Human Monoclonal Antibody
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Application 
| WB, IP |
|---|---|
| Primary Accession | P13010 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | MEM-54 |
| Calculated MW | 82705 Da |
| Purification | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
|---|---|
| Immunogen | Human T lymphocytes. |
| Shelf Life | 18 months from date of despatch. |
| Gene ID | 7520 |
| Other Names | X-ray repair cross-complementing protein 5, 3.6.4.-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2 |
| Target/Specificity | Mouse anti-Human Ku80 antibody, clone MEM-54 recognizes the 80 kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70 kDa (Ku70) and 80 kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.Mouse anti-Human Ku80 antibody, clone MEM-54 is reported as suitable for use in western blotting (Jayaram et al.2008). |
| Preservative & Stabilisers | 0.09% Sodium Azide |
| Storage | Store at +4℃ or at -20 ℃. |
| Precautions | Anti-Human Ku80 Antibody, clone MEM-54 is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.Nucleic Acids Res. 36: 5773-86. 2. Krais, A.M. et al. (2011) CHRNA5 as negative regulator of nicotine signaling in normal and cancer bronchial cells: effects on motility, migration and p63 expression.Carcinogenesis. 32 (9): 1388-95.1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins.J Radiat Res. 43 (3): 223-36.
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