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>   home   >   Products   >   Primary Antibodies   >   Anti-Human Ku80 Antibody, clone MEM-54    

Anti-Human Ku80 Antibody, clone MEM-54

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13010
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names MEM-54
Calculated MW 82705 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Human T lymphocytes.
Shelf Life 18 months from date of despatch.
Gene ID 7520
Other Names X-ray repair cross-complementing protein 5, 3.6.4.-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2
Target/Specificity Mouse anti-Human Ku80 antibody, clone MEM-54 recognizes the 80 kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70 kDa (Ku70) and 80 kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.Mouse anti-Human Ku80 antibody, clone MEM-54 is reported as suitable for use in western blotting (Jayaram et al.2008).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Ku80 Antibody, clone MEM-54 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Jayaram, S. et al. (2008) Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.
Nucleic Acids Res. 36: 5773-86. 2. Krais, A.M. et al. (2011) CHRNA5 as negative regulator of nicotine signaling in normal and cancer bronchial cells: effects on motility, migration and p63 expression.
Carcinogenesis. 32 (9): 1388-95.1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins.
J Radiat Res. 43 (3): 223-36.

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Cat# ABD11875
(40 western blots)
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