|Calculated MW||55391 Da|
|Purification||Antisera to human caspase-8 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||A 16 amino acid peptide located near the carboxy-terminus of human caspase-8 isoform A.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Caspase-8, CASP-8, 188.8.131.52, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10, CASP8, MCH5|
|Target/Specificity||ABD11880 recognises an epitope within the c-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases.Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation.Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31, see Wolf, B. B. & Green, D. R. for details.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Caspase-8 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Muzio, M. et al. (1996) FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.Cell 85: 817 - 827.2. Wolf, B. B. and Green, D. R. (1999) Suicidal tendencies: apoptotic cell death by caspase family proteinases.J. Biol. Chem. 274: 20049 - 20052.3. Cohen, G. M. (1997) Caspases: the executioners of apoptosis.Biochem. J. 326: 1 - 16.
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