|Application ||WB, IHC-P, IHC-F, IF, E|
|Calculated MW||30515 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human mast cell tryptase purified from human lung tissue.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tryptase alpha/beta-1, Tryptase-1, 126.96.36.199, Tryptase I, Tryptase alpha-1, TPSAB1, TPS1, TPS2, TPSB1|
|Target/Specificity||Mouse anti-Human Mast Cell Tryptase, clone AA1 recognizes human mast cell tryptase, both alpha and beta isoforms.Mouse anti-Mast cel tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Wallset al.1990).Tryptases are the products of a number of genes and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Mast Cell Tryptase Antibody, clone AA1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Walls, A.F. et al. (1990) Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase.J Pathol. 162 (2): 119-26. 2. Walls, A.F. et al. (1990) Production and characterization of monoclonal antibodies specific for human mast cell tryptase.Clin Exp Allergy. 20 (5): 581-9. 3. Ozaki, K. et al. (2002) Mast cell tumors of the gastrointestinal tract in 39 dogs.Vet Pathol. 39 (5): 557-64. 4. Louiset, E. et al. (2008) Ectopic expression of serotonin7 receptors in an adrenocortical carcinoma co-secreting renin and cortisol. Endocr Relat Cancer.15: 1025-34. 5. Xiang, M. et al. (2011) Usefulness of serum tryptase level as an independent biomarker for coronary plaque instability in a Chinese population.Atherosclerosis. 215 (2): 494-9. 6. Jacob, C. et al. (2005) Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.J Biol Chem. 280: 31936-48. 7. Mauro, L.V. et al. (2008) Association between mast cells of different phenotypes and angiogenesis in colorectal cancer.Mol Med Report. 1: 895-902. 8. Liu, J. et al. (2009) Genetic deficiency and pharmacological stabilization of mast cells reduce diet-induced obesity and diabetes in mice.Nat Med. 15: 940-5. 9. Asano-Kato, N. et al. (2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2.Invest Ophthalmol Vis Sci. 46: 4622-6. 10. Facoetti, A. et al. (2006) Histochemical study of cardiac mast cells degranulation and collagen deposition: interaction with the cathecolaminergic system in the rat.Eur J Histochem. 50: 133-40. 11. Thienemann, F. et al. (2004) Regulation of mast cell characteristics by cytokines: divergent effects of interleukin-4 on immature mast cell lines versus mature human skin mast cells.Arch Dermatol Res. 296: 134-8. 12. Dichlberger, A. et al. (2011) Lipid body formation during maturation of human mast cells.J Lipid Res. 52: 2198-208. 13. Kazama, I. et al. (2015) Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.Nephrology (Carlton). 20 (9): 609-16. 14. Sevestre, H.P. et al. (2015) Mast Cell and Follicular Lymphoma, Association or Relationship.Abstract 338 -Hematol Oncol. 33 Suppl 1: 263. 15. Kawarai, S. et al. (2010) Cultivation and characterization of canine skin-derived mast cells.J Vet Med Sci. 72 (2): 131-40. 16. Perbellini, O. et al. (2011) Primary role of multiparametric flow cytometry in the diagnostic work-up of indolent clonal mast cell disorders.Cytometry B Clin Cytom. 80 (6): 362-8. 17. Baba, A. et al. (2016) Less Contribution of Mast Cells to the Progression of Renal Fibrosis in Rat Kidneys with Chronic Renal Failure.Nephrology (Carlton). Feb 2. [Epub ahead of print]
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